Dataset Information

Phosphoproteomic analysis of lettuce (Lactuca sativa L.) reveals starch and sucrose metabolism functions during bolting induced by high temperature.

ABSTRACT: High temperatures induce early bolting in lettuce (Lactuca sativa L.), which decreases both quality and production. However, knowledge of the molecular mechanism underlying high temperature promotes premature bolting is lacking. In this study, we compared lettuce during the bolting period induced by high temperatures (33/25 °C, day/night) to which raised under controlled temperatures (20/13 °C, day/night) using iTRAQ-based phosphoproteomic analysis. A total of 3,814 phosphorylation sites located on 1,766 phosphopeptides from 987 phosphoproteins were identified after high-temperature treatment,among which 217 phosphoproteins significantly changed their expression abundance (116 upregulated and 101 downregulated). Most phosphoproteins for which the abundance was altered were associated with the metabolic process, with the main molecular functions were catalytic activity and transporter activity. Regarding the functional pathway, starch and sucrose metabolism was the mainly enriched signaling pathways. Hence, high temperature influenced phosphoprotein activity, especially that associated with starch and sucrose metabolism. We suspected that the lettuce shorten its growth cycle and reduce vegetative growth owing to changes in the contents of starch and soluble sugar after high temperature stress, which then led to early bolting/flowering. These findings improve our understanding of the regulatory molecular mechanisms involved in lettuce bolting.

SUBMITTER: Qin X

PROVIDER: S-EPMC7771692 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Phosphoproteomic analysis of lettuce (Lactuca sativa L.) reveals starch and sucrose metabolism functions during bolting induced by high temperature.

Qin Xiaoxiao X Li Panpan P Lu Shaowei S Sun Yanchuan Y Meng Lifeng L Hao Jinghong J Fan Shuangxi S

PloS one 20201229 12

High temperatures induce early bolting in lettuce (Lactuca sativa L.), which decreases both quality and production. However, knowledge of the molecular mechanism underlying high temperature promotes premature bolting is lacking. In this study, we compared lettuce during the bolting period induced by high temperatures (33/25 °C, day/night) to which raised under controlled temperatures (20/13 °C, day/night) using iTRAQ-based phosphoproteomic analysis. A total of 3,814 phosphorylation sites located ...[more]

PMID: 33373388

Similar Datasets

Project description:Temperature plays an important role in the growth and development of lettuce (Lactuca sativa L.). A high temperature can induce bolting, which decreases both the quality and production of lettuce. However, knowledge of underlying lettuce bolting is still lacking, especially in the molecular basis that high temperature promotes the premature bolting of lettuce at the phosphorylated level. In this study, we analyzed the lettuce stems during the bolting period induced by a high temperature (33oC/25oC, day/ night) and a controlled temperature (20oC/13oC, day/night) using iTRAQ-based phosphoproteome and 987 phosphoproteins were significantly changed after high-temperature treatment and a total of 3814 phosphorylation sites located in 1766 phosphopeptides were identified. Also, we found 216 phosphoproteins significantly regulated, of which 116 up-regulated and 101 down-regulated, and the abundance changed peptides after high-temperature treatment was significantly involved in the biological process (BP) of water homeostasis. Most of these phosphoproteins were associated with metabolic process, correspondingly the main molecular functions are catalytic activity and transporter activity. The top 10 signaling pathways included spliceosome, starch and sucrose metabolism, RNA transport, protein processing in endoplasmic reticulum, phosphatidylinositol signaling system, phospholipase D signaling pathway, apoptosis, protein export, tight junction, and glycerolipid metabolism. For further, we found the high-temperature influence phosphoprotein activity associated with metabolism, especially, starch and sucrose metabolism, glycerolipid metabolism, methane metabolism. The kinases, including proline-directed, acidic, basic, others and tyrosine, were predicted to be involved in signal transduction and metabolism regulation. The proline-directed motif such as ..SP..[s]P, [s]P, [t]P was the dominant class, proline-directed motifs associated with MAK kinase, and several acidic motifs are casein kinase II motif are necessary for lettuce blotting. Our study showed phosphoproteome profiling in response to high temperature and provides a better understanding of the molecular mechanisms regulated in lettuce blotting

Project description:Bolting is a key process in the growth and development of lettuce (Lactuca sativa L.). High temperature can induce earlier bolting which decreases in both quality and production of lettuce. However, knowledge underlying lettuce bolting is still lacking. To better understand the molecular basis of bolting, a comparative proteomics analysis was conducted on lettuce stems in the bolting period induced by high temperature (33 °C) compared with a control (20 °C) using iTRAQ-based proteomics, phenotypic measures, and biological verifications. High temperature induced lettuce bolting, while control temperature did not. Of the 6656 proteins identified, 758 proteins significantly altered their expression level induced by high-temperature relative to the control, of which 409 were up-regulated and 349 down-regulated. Proteins with abundance level change were mainly involved in photosynthesis, carbohydrate metabolism, stress response, hormone synthesis, and signal transduction. These differential proteins were mainly enriched in pathways associated with photosynthesis and tryptophan metabolism involving in auxin (IAA) biosynthesis. Among the differentially expressed proteins associated with photosynthesis and tryptophan metabolism were up-regulated. Moreover, in gibberellin (GA) biosynthesis pathway, 10 of main enzymes of P450 were up-regulated. Proteins related to SAUR and GRP, implicated in IAA and GA signal transduction were up-regulated, and the phosphorylation and ubiquitination related proteins regulating IAA and GA signal transduction were also induced. These findings indicate that a high temperature enhances the function of photosynthesis, IAA and GA synthesis and signal transduction to promote the process of bolting, which is in line with the physiology and transcription levels of IAA and GA metabolism. Our data provide a first comprehensive dataset for gaining novel understanding of the molecular basis underlying lettuce bolting induced by high temperature. It is potentially important for further functional analysis and genetic manipulation for molecular breeding to breed new cultivar of lettuce to restrain early bolting, which is vital for improving vegetable quality.

Dataset Information

Phosphoproteomic analysis of lettuce (Lactuca sativa L.) reveals starch and sucrose metabolism functions during bolting induced by high temperature.

Publications

Phosphoproteomic analysis of lettuce (Lactuca sativa L.) reveals starch and sucrose metabolism functions during bolting induced by high temperature.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure