Dataset Information

Fruit quality and antioxidant potential of Prunus humilis Bunge accessions.

ABSTRACT: In this study, we aimed to evaluate the fruit quality of Prunus humilis and identify cultivars that could provide superior human health benefits. We measured the basic characteristics, bioactive compounds, and antioxidant capacities of 137 P. humilis accessions. Flavonoid and phenol content were determined via colorimetry and ultrahigh performance liquid chromatography. Single fruit and stone weights varied widely and were genetically diverse among accessions. The variation in soluble solid content was comparatively narrow. Total flavonoid content (TFC) ranged from 3.90 to 28.37 mg/g FW, with an average of 10.58 mg/g FW in 2019. Significant differences between accessions in terms of TFC, total phenol content, and antioxidant capacity were found. TFC in the accessions was normally distributed and predominantly in the medium range (9.57-15.23 mg/g FW). Red was the predominant peel color over all other phenotypes (i.e., dark red, red, light red, red-orange, and yellow). There was no obvious correlation between peel color and TFC. Catechin was the major flavonoid component in the fruit. Principal component analysis showed that TFC, ABTS, single fruit weight, and vertical and horizontal diameter contributed to the first two principal components for each accession. Accessions 10-02, 3-17-2, 3-17-4, and JD1-6-7-37 were characterized by high TFC, ABTS, and large fruit. We believe that our results will aid in the breeding and functional food processing of Prunus humilis.

SUBMITTER: Fu H

PROVIDER: S-EPMC7773198 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Fruit quality and antioxidant potential of Prunus humilis Bunge accessions.

Fu Hongbo H Mu Xiaopeng X Wang Pengfei P Zhang Jiancheng J Fu Baochun B Du Junjie J

PloS one 20201230 12

In this study, we aimed to evaluate the fruit quality of Prunus humilis and identify cultivars that could provide superior human health benefits. We measured the basic characteristics, bioactive compounds, and antioxidant capacities of 137 P. humilis accessions. Flavonoid and phenol content were determined via colorimetry and ultrahigh performance liquid chromatography. Single fruit and stone weights varied widely and were genetically diverse among accessions. The variation in soluble solid cont ...[more]

PMID: 33378359

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Project description:Dynamic changes in flavonoid, total phenol, and antioxidant potential in different Prunus humilis accessions during fruit development stages were studied in order to provide a reference for the optimum harvest time for flavonoid extraction. 'Nongda 4', 'Nongda 5', 'DS-1' and '02-16' were selected as plant materials to determine the content of flavonoid, total phenol and antioxidant indices during six fruit development stages. Changes in total flavonoid content (TFC) and total phenol content (TPC) in different accessions of P. humilis were slightly different depending on the development stage of P. humilis fruit. TFC and TPC in 'Nongda 5' fruit showed a trend of continuous decline. There was a small increase in TFC and TPC from the young fruit stage to the stone hardening stage, followed by a decreasing trend, and then to the lowest level at the ripening stage of 'Nongda 4', 'DS-1', and '02-16' fruits. The trend of antioxidant capacity (ABTS, FRAP, DPPH) with the TFC and TPC of P. humilis fruit was basically the same, and the correlation analysis results showed that the TFC of P. humilis fruit was positively correlated with the antioxidant indices (P<0.01). Catechin (CC), rutin (RT), and quercetin-7-O-β-D-glucopyranoside (Q7G) were detected in all the fruit development stages of the four P. humilis fruits. Among them, catechin was the most abundant component, accounting for approximately 10%. Myricetin (MC) and quercetin (QC) were generally detected only in the early fruit development stage, but not in the later fruit development stage. Correlation analysis showed that the flavonoid components with TFC, TPC, and antioxidant indices differed between the different accessions. RT, CC, and liquiritigenin (LR) had a stronger correlation with TFC and antioxidant indices. Cyanidin-3-O-glucoside (C3G) was not detected until the coloring stage in two red P. humilis accessions ('Nongda 4' and 'DS-1'), and so it is better to choose a red P. humilis fruit to extract C3G at the ripening stage. Selecting an early stage of fruit development, especially the stone hardening stage, was important for extracting flavonoids, total phenols and other components. We believe that our results will provide basic information and reference for evaluation of fruit nutrition and health benefits, breeding of functional new varieties, and efficient utilization of P. humilis fruit.

Project description:BackgroundPrunus fruit development, growth, ripening, and senescence includes major biochemical and sensory changes in texture, color, and flavor. The genetic dissection of these complex processes has important applications in crop improvement, to facilitate maximizing and maintaining stone fruit quality from production and processing through to marketing and consumption. Here we present an integrated fruit quality gene map of Prunus containing 133 genes putatively involved in the determination of fruit texture, pigmentation, flavor, and chilling injury resistance.ResultsA genetic linkage map of 211 markers was constructed for an intraspecific peach (Prunus persica) progeny population, Pop-DG, derived from a canning peach cultivar 'Dr. Davis' and a fresh market cultivar 'Georgia Belle'. The Pop-DG map covered 818 cM of the peach genome and included three morphological markers, 11 ripening candidate genes, 13 cold-responsive genes, 21 novel EST-SSRs from the ChillPeach database, 58 previously reported SSRs, 40 RAFs, 23 SRAPs, 14 IMAs, and 28 accessory markers from candidate gene amplification. The Pop-DG map was co-linear with the Prunus reference T x E map, with 39 SSR markers in common to align the maps. A further 158 markers were bin-mapped to the reference map: 59 ripening candidate genes, 50 cold-responsive genes, and 50 novel EST-SSRs from ChillPeach, with deduced locations in Pop-DG via comparative mapping. Several candidate genes and EST-SSRs co-located with previously reported major trait loci and quantitative trait loci for chilling injury symptoms in Pop-DG.ConclusionThe candidate gene approach combined with bin-mapping and availability of a community-recognized reference genetic map provides an efficient means of locating genes of interest in a target genome. We highlight the co-localization of fruit quality candidate genes with previously reported fruit quality QTLs. The fruit quality gene map developed here is a valuable tool for dissecting the genetic architecture of fruit quality traits in Prunus crops.

Dataset Information

Fruit quality and antioxidant potential of Prunus humilis Bunge accessions.

Publications

Fruit quality and antioxidant potential of Prunus humilis Bunge accessions.

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