HIV proviral DNA integration can drive T cell growth ex vivo.
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ABSTRACT:
SUBMITTER: Yoon JK
PROVIDER: S-EPMC7777207 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Publications
HIV proviral DNA integration can drive T cell growth ex vivo.
Yoon John K JK Holloway Joseph R JR Wells Daria W DW Kaku Machika M Jetton David D Brown Rebecca R Coffin John M JM
Proceedings of the National Academy of Sciences of the United States of America 20201214 52
In vivo clonal expansion of HIV-infected T cells is an important mechanism of viral persistence. In some cases, clonal expansion is driven by HIV proviral DNA integrated into one of a handful of genes. To investigate this phenomenon in vitro, we infected primary CD4+ T cells with an HIV construct expressing GFP and, after nearly 2 mo of culture and multiple rounds of activation, analyzed the resulting integration site distribution. In each of three replicates from each of two donors, we detected ...[more]
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