Dataset Information

Conversion of Rutin, a Prevalent Dietary Flavonol, by the Human Gut Microbiota.

ABSTRACT: The gut microbiota plays a pivotal role in the conversion of dietary flavonoids, which can affect their bioavailability and bioactivity and thereby their health-promoting properties. The ability of flavonoids to metabolically-activate the microbiota has, however, not been systematically evaluated. In the present study, we used a fluorescence-based single-cell activity measure [biorthogonal non-canonical ammino acid-tagging (BONCAT)] combined with fluorescence activated cell sorting (FACS) to determine which microorganisms are metabolically-active after amendment of the flavonoid rutin. We performed anaerobic incubations of human fecal microbiota amended with rutin and in the presence of the cellular activity marker L-azidohomoalanine (AHA) to detect metabolically-active cells. We found that 7.3% of cells in the gut microbiota were active after a 6 h incubation and 26.9% after 24 h. We then sorted BONCAT-positive cells and observed an enrichment of Lachnospiraceae (Lachnoclostridium and Eisenbergiella), Enterobacteriaceae, Tannerellaceae, and Erysipelotrichaceae species in the rutin-responsive fraction of the microbiota. There was marked inter-individual variability in the appearance of rutin conversion products after incubation with rutin. Consistent with this, there was substantial variability in the abundance of rutin-responsive microbiota among different individuals. Specifically, we observed that Enterobacteriaceae were associated with conversion of rutin into quercetin-3-glucoside (Q-glc) and Lachnospiraceae were associated with quercetin (Q) production. This suggests that individual microbiotas differ in their ability to metabolize rutin and utilize different conversion pathways.

SUBMITTER: Riva A

PROVIDER: S-EPMC7779528 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Conversion of Rutin, a Prevalent Dietary Flavonol, by the Human Gut Microbiota.

Riva Alessandra A Kolimár Ditta D Spittler Andreas A Wisgrill Lukas L Herbold Craig W CW Abrankó László L Berry David D

Frontiers in microbiology 20201221

The gut microbiota plays a pivotal role in the conversion of dietary flavonoids, which can affect their bioavailability and bioactivity and thereby their health-promoting properties. The ability of flavonoids to metabolically-activate the microbiota has, however, not been systematically evaluated. In the present study, we used a fluorescence-based single-cell activity measure [biorthogonal non-canonical ammino acid-tagging (BONCAT)] combined with fluorescence activated cell sorting (FACS) to det ...[more]

PMID: 33408702

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Dataset Information

Conversion of Rutin, a Prevalent Dietary Flavonol, by the Human Gut Microbiota.

Publications

Conversion of Rutin, a Prevalent Dietary Flavonol, by the Human Gut Microbiota.

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