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High resolution single particle Cryo-EM refinement using JSPR.

ABSTRACT: JSPR is a single particle cryo-EM image processing and 3D reconstruction software developed in the Jiang laboratory at Purdue University. It began as a few refinement scripts for symmetric and asymmetric reconstructions of icosahedral viruses, but has grown into a comprehensive suite of tools for building ab initio reconstructions, high resolution refinements of viruses, protein complexes of arbitrary symmetries including helical tubes/filaments, and image file handling utilities. In this review, we will present examples achieved using JSPR and demonstrate recently implemented features of JSPR such as multi-aberration "alignments" and automatic optimization of masking for the assessment of map resolution using "true" FSC.

SUBMITTER: Sun C

PROVIDER: S-EPMC7779749 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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High resolution single particle Cryo-EM refinement using JSPR.

Sun Chen C Gonzalez Brenda B Vago Frank S FS Jiang Wen W

Progress in biophysics and molecular biology 20200703

JSPR is a single particle cryo-EM image processing and 3D reconstruction software developed in the Jiang laboratory at Purdue University. It began as a few refinement scripts for symmetric and asymmetric reconstructions of icosahedral viruses, but has grown into a comprehensive suite of tools for building ab initio reconstructions, high resolution refinements of viruses, protein complexes of arbitrary symmetries including helical tubes/filaments, and image file handling utilities. In this review ...[more]

PMID: 32622834

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