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The method to dynamically screen and print single cells using microfluidics with pneumatic microvalves

ABSTRACT: Printing single cells into individual chambers is of critical importance for single-cell analysis using traditional equipment, for instance, single-cell clonal expansion or sequencing. The size of cells can usually be a reflection of their types, functions, and even cell cycle phases. Therefore, printing individual cells within the desired size range is of essential application potential in single-cell analysis. This paper presents a method for the development of a microfluidic chip integrating pneumatic microvalves to print single cells with appropriate size into standard well plates. The reported method provided essential guidelines for the fabrication of multi-layer microfluidic chips, control of the membrane deflection to screen cell size, and printing of single cells. In brief, this paper reports:• the manufacturing of the chip using standard soft lithography;• the protocol to dynamically screen both the lower and the upper size limit of cells passing through the valves by deflection of the valve membrane;• the screening and dispensing of suspended human umbilical vein endothelial cells (HUVECs) into 384-well plates with high viability. Graphical abstract Image, graphical abstract

SUBMITTER: Chen C

PROVIDER: S-EPMC7779779 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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