Dataset Information
CASC8 lncRNA Promotes the Proliferation of Retinoblastoma Cells Through Downregulating miR34a Methylation.
Ontology highlight
ABSTRACT: Background
CASC8 lncRNA has been proven to be oncogenic in a variety of cancers, but its role in other types of cancer remains unclear. This study was to investigate the role of CASC8 in retinoblastoma (Rb).Methods
RT-qPCR was performed to determine the expression of CASC8 and miR34a in paired Rb and nontumor tissue. Overexpression of CASC8 and miR34a in Rb cells was achieved to evaluate the interaction between them. Methylation-specific PCR was used to analyze the effect of CASC8 overexpression on MIR34 A gene methylation. CCK8 assays were used to analyze cell proliferation.Results
The results showed that CASC8 expression was upregulated and miR34a expression downregulated in Rb tissue. Moreover, miR34a expression was negatively correlated with the of CASC8 expression in Rb tissue. Overexpression of CASC8 decreased expression of miR34a and increased methylation of MIR 34A in Rb cells. In addition, overexpression of CASC8 reduced the inhibitory effects of miR34a on Rb-cell proliferation.Conclusion
CASC8 may promote Rb cell proliferation by downregulating miR34a methylation.
SUBMITTER: Yang B
PROVIDER: S-EPMC7779858 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Publications
<i>CASC8</i> lncRNA Promotes the Proliferation of Retinoblastoma Cells Through Downregulating miR34a Methylation.
Cancer management and research 20201230
<h4>Background</h4><i>CASC8</i> lncRNA has been proven to be oncogenic in a variety of cancers, but its role in other types of cancer remains unclear. This study was to investigate the role of <i>CASC8</i> in retinoblastoma (Rb).<h4>Methods</h4>RT-qPCR was performed to determine the expression of <i>CASC8</i> and miR34a in paired Rb and nontumor tissue. Overexpression of <i>CASC8</i> and miR34a in Rb cells was achieved to evaluate the interaction between them. Methylation-specific PCR was used t ...[more]