Using LC-MS to Identify Clipping in Self-Assembled Nanoparticles During Vaccine Development.
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ABSTRACT:
SUBMITTER: Schneck NA
PROVIDER: S-EPMC7780558 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
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Using LC-MS to Identify Clipping in Self-Assembled Nanoparticles During Vaccine Development.
Schneck Nicole A NA Ivleva Vera B VB Rosales-Zavala Erwin E Wang Xiangchun X Gollapudi Deepika D Cooper Jonathan W JW Lei Q Paula QP
Journal of the American Society for Mass Spectrometry 20191008 12
A hemagglutinin stabilized stem nanoparticle (HA-SS-np) that is designed to provide broad protection against influenza is being developed as a potential vaccine. During an early formulation screening study, reducing gel (rCGE) analysis indicated product degradation in a few candidate buffers at the first-week accelerated stability point, whereas no change was shown in the size exclusion chromatography (SEC) measurement. A LC-MS workflow was therefore applied to investigate the integrity of this ...[more]
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