Effects of brewers' spent grain protein hydrolysates on gas production, ruminal fermentation characteristics, microbial protein synthesis and microbial community in an artificial rumen fed a high grain diet.

ABSTRACT:

Background

Brewers' spent grain (BSG) typically contains 20%?-?29% crude protein (CP) with high concentrations of glutamine, proline and hydrophobic and non-polar amino acid, making it an ideal material for producing value-added products like bioactive peptides which have antioxidant properties. For this study, protein was extracted from BSG, hydrolyzed with 1% alcalase and flavourzyme, with the generated protein hydrolysates (AlcH and FlaH) showing antioxidant activities. This study evaluated the effects of AlcH and FlaH on gas production, ruminal fermentation characteristics, nutrient disappearance, microbial protein synthesis and microbial community using an artificial rumen system (RUSITEC) fed a high-grain diet.

Results

As compared to the control of grain only, supplementation of FlaH decreased (P 3-N and decreased (P 2 production. Supplementation of FlaH decreased (P 4 in total gas and dissolved-CH₄ (dCH₄) in dissolved gas. Addition of monensin reduced (P 4 and H₂ emissions. Total microbial nitrogen production was decreased (P ConclusionsThe supplementation of FlaH in high-grain diets may potentially protect CP and starch from ruminal degradation, without adversely affecting fibre degradation and VFA profiles. It also showed promising effects on reducing CH₄ production by suppressing H₂ production. Protein enzymatic hydrolysates from BSG using flavourzyme showed potential application to high value-added bio-products.

SUBMITTER: Ran T 

PROVIDER: S-EPMC7780661 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature