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Mitochondrial genome of Bactrocera tuberculata using next-generation sequencing from China and its phylogenetic implication.

ABSTRACT: The complete mitochondrial genome (mitogenome) of Bactrocera tuberculata (Diptera: Tephritidae: Dacinae) was sequenced and annotated. The mitochondrial genome is 15,273 bp (GenBank No. MT196006), containing 72.2% A + T (A 38.4%, C 16.9%, G 10.9%, and T 33.8%), which is the classical structure for insect mitogenome. All PCGs were started with ATN (ATA/ATG/ATT/ATC) and terminated with TAA except ND3, which ends with TAG. Additionally, the phylogenetic tree confirmed that B. tuberculata was closely related to Bactrocera diaphora and Bactrocera oleae. The current study would enrich the information about mitogenomes of the fruit flies.

SUBMITTER: Wang T

PROVIDER: S-EPMC7781919 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Mitochondrial genome of Bactrocera tuberculata using next-generation sequencing from China and its phylogenetic implication.

Wang Tao T Ren Yanling Y Liu Zhongshan Z Lei Quwen Q Tang Jian J

Mitochondrial DNA. Part B, Resources 20200611 3

The complete mitochondrial genome (mitogenome) of Bactrocera tuberculata (Diptera: Tephritidae: Dacinae) was sequenced and annotated. The mitochondrial genome is 15,273 bp (GenBank No. MT196006), containing 72.2% A + T (A 38.4%, C 16.9%, G 10.9%, and T 33.8%), which is the classical structure for insect mitogenome. All PCGs were started with ATN (ATA/ATG/ATT/ATC) and terminated with TAA except ND3, which ends with TAG. Additionally, the phylogenetic tree confirmed that B. tuberculata</ ...[more]

PMID: 33457816

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Project description:BackgroundAccumulating evidence implicates mitochondrial DNA (mtDNA) alleles, which are independent of the nuclear genome, in disease, especially in human metabolic diseases. However, this area of investigation has lagged behind in researching the nuclear alleles in complex traits, for example, in gout.MethodsNext-generation sequencing was utilized to investigate the relationship between mtDNA alleles and phenotypic variations in 52 male patients with gout and 104 age-matched male non-gout controls from the Taiwan Biobank whole-genome sequencing samples. Differences from a reference sequence (GRCh38) were identified. The sequence kernel association test (SKAT) was applied to identify gout-associated alleles in mitochondrial genes. The tools Polymorphism Phenotyping, Sorting Intolerant From Tolerant (SIFT), Predict the pathology of Mutations (PMUT), Human Mitochondrial Genome Database (mtDB), Multiple Alignment using Fast Fourier Transform (MAFFT), and Mammalian Mitochondrial tRNA Genes (Mamit-tRNA) were used to evaluate pathogenicity of alleles. Validation of selected alleles by quantitative polymerase chain reaction of single nucleotide polymorphisms (qPCR SNPs) was also performed.ResultsWe identified 456 alleles in patients with gout and 640 alleles in non-gout controls with 274 alleles shared by both. Mitochondrial genes were associated with gout, with MT-CO3, MT-TA, MT-TC, and MT-TT containing potentially pathogenic gout-associated alleles and displaying evidence of gene-gene interactions. All heteroplasmy levels of potentially pathogenic alleles exceeded metabolic thresholds for pathogenicity. Validation assays confirmed the next-generation sequencing results of selected alleles. Among them, potentially pathogenic MT-CO3 alleles correlated with high-density lipoprotein (HDL) levels (P = 0.034).ConclusionThis study provided two scientific insights. First, this was the most extensive mitochondrial genomic profiling associated with gout. Second, our results supported the roles of mitochondria in gout and HDL, and this comprehensive analysis framework can be applied to other diseases in which mitochondrial dysfunction has been implicated.

Dataset Information

Mitochondrial genome of Bactrocera tuberculata using next-generation sequencing from China and its phylogenetic implication.

Publications

Mitochondrial genome of <i>Bactrocera tuberculata u</i>sing next-generation sequencing from China and its phylogenetic implication.

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