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The complete mitochondrial genome of Asparagus officinalis L.

ABSTRACT: Asparagus officinalis L. is a horticultural plant in nature and belongs to the genus Asparagus of Asparagaceae family. The complete mitochondrial (mt) genome of A. officinalis was 492,062 bp in length with a 45.9% GC content. A total of 60 genes were annotated including 37 protein-coding genes, 17 tRNA genes, and 6 rRNA genes. Phylogenetic tree analysis using the neighbor-joining method demonstrated that A. officinalis was most closely related to Allium cepa and separated from Beta vulgaris subsp. Vulgaris based on the complete mt genome sequence.

SUBMITTER: Sheng W

PROVIDER: S-EPMC7782815 | biostudies-literature |

REPOSITORIES: biostudies-literature

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The garden asparagus (<i>Asparagus officinalis</i> L.) mitochondrial genome revealed rich sequence variation throughout whole sequencing data.

Project description:Garden asparagus (Asparagus officinalis L.) is a horticultural crop with high nutritional and medical value, considered an ideal plant for sex determination research among many dioecious plants, whose genomic information can support genetic analysis and breeding programs. In this research, the entire mitochondrial genome of A. officinalis was sequenced, annotated and assembled using a mixed Illumina and PacBio data. The garden asparagus circular mitochondrial genome measures 492,062 bp with a GC value of 45.9%. Thirty-six protein-coding genes, 17 tRNA and 6 rRNA genes were annotated, among which 8 protein-coding genes contained 16 introns. In addition, 254 SSRs with 10 complete tandem repeats and 293 non-tandem repeats were identified. It was found that the codons of edited sites located in the amino acids showed a leucine-formation trend, and RNA editing sites mainly caused the mutual transformation of amino acids with the same properties. Furthermore, 72 sequence fragments accounting for 20,240 bp, presentating 4.11% of the whole mitochondrial genome, were observed to migrate from chloroplast to mitochondrial genome of A. officinalis. The phylogenetic analysis showed that the closest genetic relationship between A. officinalis with onion (Allium cepa) inside the Liliaceae family. Our results demonstrated that high percentage of protein-coding genes had evolutionary conservative properties, with Ka/Ks values less than 1. Therefore, this study provides a high-quality garden asparagus mitochondrial genome, useful to promote better understanding of gene exchange between organelle genomes.

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Data of whole genome sequencing of five garden asparagus (Asparagus officinalis) individuals with the MinION nanopore sequencer.

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Genome-Wide Identification and Analysis of the NAC Transcription Factor Gene Family in Garden Asparagus (<i>Asparagus officinalis</i>).

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