Dataset Information

Alanylglutamine Relieved Asthma Symptoms by Regulating Gut Microbiota and the Derived Metabolites in Mice.

ABSTRACT:

Objective

Allergic asthma is a chronic inflammatory disease, which seriously affects the life quality of patients, especially children. Alanylglutamine is a nutritional supplement with potential protective and anti-inflammatory effects, but its function in allergic asthma remains elusive. In this study, we focused on the investigations of the roles and functional mechanism of Alanylglutamine in asthma.

Methods

Ovalbumin (OVA) induction was utilized to establish a mouse asthma model. 16S rDNA sequencing was performed to compare the diversity of intestinal microorganisms under different treatments. Gas chromatography was utilized to screen the intestinal microbe-short-chain fatty acids in the stool. The lung tissue was extracted to determine signaling pathways, including AMPK, NF-?B, mTOR, STAT3, IKK?, TGF-?, and IL-1? through Western blot or RT-qPCR.

Results

It was observed that Alanylglutamine reduced the cytokine in OVA-induced allergic asthma mice. H&E staining showed obvious pneumonia symptoms in the asthma group, while Alanylglutamine alleviated the inflammatory infiltration. Alanylglutamine reversed gut microbiota compositions in OVA-induced allergic asthma mice and enhanced the butyric acid level. The protective role of Alanylglutamine may be associated with the gut microbiota-butyric acid-GPR43 pathway in asthma mice. In contrast to the OVA group, Alanylglutamine activated the protein expression of P-AMPK/AMPK and inhibited the protein expression of P-mTOR/mTOR, P-P65/P65, P-STAT3/STAT3, P-IKK?/IKK?, TGF-?, and IL-1?, with similar effects from butyric acid.

Conclusion

The results indicated that Alanylglutamine might be beneficial for asthma, and its effect was achieved through the regulation on microbiota and the derived metabolites. The therapeutic effects might be associated with AMPK, NF-?B, mTOR, and STAT3 signaling pathways. These findings will help identify effective therapeutic direction to alleviate allergic inflammation of the lungs and airways.

SUBMITTER: Liu SK

PROVIDER: S-EPMC7785351 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Publications

Alanylglutamine Relieved Asthma Symptoms by Regulating Gut Microbiota and the Derived Metabolites in Mice.

Liu Shao-Kun SK Ma Li-Bing LB Yuan Yu Y Ji Xiao-Ying XY Sun Wen-Jin WJ Duan Jia-Xi JX Zeng Qing-Ping QP Wasti Binaya B Xiao Bing B Zheng Jian-Fei JF Chen Ping P Xiang Xu-Dong XD

Oxidative medicine and cellular longevity 20201229

<h4>Objective</h4>Allergic asthma is a chronic inflammatory disease, which seriously affects the life quality of patients, especially children. Alanylglutamine is a nutritional supplement with potential protective and anti-inflammatory effects, but its function in allergic asthma remains elusive. In this study, we focused on the investigations of the roles and functional mechanism of Alanylglutamine in asthma.<h4>Methods</h4>Ovalbumin (OVA) induction was utilized to establish a mouse asthma mode ...[more]

PMID: 33456673

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Dataset Information

Alanylglutamine Relieved Asthma Symptoms by Regulating Gut Microbiota and the Derived Metabolites in Mice.

Objective

Methods

Results

Conclusion

Publications

Alanylglutamine Relieved Asthma Symptoms by Regulating Gut Microbiota and the Derived Metabolites in Mice.

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