The Effects of Hypoxia-Reoxygenation in Mouse Digital Flexor Tendon-Derived Cells.

ABSTRACT:

Objective

Ischemia-reperfusion injury refers to the exacerbated and irreversible tissue damage caused by blood flow restoration after a period of ischemia. The hypoxia-reoxygenation (H/R) model in vitro is ideal for studying ischemia-reperfusion injury at the cellular level. We employed this model and investigated the effects of cobalt chloride- (CoCl₂-) induced H/R in cells derived from mouse digital flexor tendons.

Materials and methods

Various H/R conditions were simulated via treatment of tendon-derived cells with different concentrations of CoCl₂ for 24 h, followed by removal of CoCl₂ to restore a normal oxygen state for up to 96 h. Cell viability was measured using the Cell Counting Kit-8 (CCK-8) assay. Cell growth was determined via ob

SUBMITTER: Chen C 

PROVIDER: S-EPMC7787768 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature