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Acute and repeated-dose toxicity of Echinops kebericho Mesfin essential oil

ABSTRACT: Graphical abstract Highlights • No mortality was observed up to 2000 mg/kg dose in acute toxicity study of Echinops kebericho essential oil.• On repeated-dose toxicity study, essential oil treated groups and controls did not show significant variations.• No significant variation was observed in food consumption, body and organ weight, and histopathology between treated and control groups.• Urea, albumin, aspartate aminotransferase, andorgan weight of kidney significantly varied between some treated groups compared to control.• Significant variations were observed between male and female in many parameters. Echinops kebericho Mesfin is used for the management of various diseases and fumigation during child birth. This study investigated acute and repeated-dose toxicity of E. kebericho M. essential oils (EOs). The study was conducted in Swiss albino mice. Organ weight, histopathology and clinical chemistry were analyzed. The dose and duration of treatment were defined in accordance with Organization for Economic Co-operation and Development (OECD) guideline. No mortality was observed in acute oral dose toxicity study up to 2000 mg/kg per body weight. Compared to control group, treated groups did not show significant abnormalities in body weight and most parameters of clinical chemistry parameters and relative organ weight in repeated-dose toxicity study. However, urea, albumin, aspartate aminotransferase, and relative organ weight of right kidney showed variations in treated groups compared to control group. All treated groups and control group showed normal histology except lymphocytic infiltrates observed on the kidney with 200 mg/kg treated female group. The current study revealed that EO of E. kebericho M. could be considered well tolerated in acute and repeated-dose exposure. Further, teratogenic, mutagenic, carcinogenic, and sub-chronic and chronic toxicity studies are warranted.

SUBMITTER: Deyno S

PROVIDER: S-EPMC7787995 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Project description:BACKGROUND:Echinops kebericho is widely used for treatment of a variety of diseases including infectious, non-infectious disease and fumigation during child birth. Antibacterial, antimalarial, anti-leshimania, anti-diarrheal and insect repellent activities have been elucidated. Its toxicity profile is not yet investigated and thus this study was to investigate acute and sub-acute toxicity of E. kebericho decoctions. METHODS:Acute toxicity study was performed in female Wistar albino rats with single oral dose and followed up to 14 days. The sub-acute oral dose toxicity studies were conducted in rats of both sexes in accordance with the repeated dose 28-day oral toxicity study in rodent OECD guidelines. Physical observations were made regularly during the study period while body weight was measured weekly. Organ weight, histopathology, clinical chemistry and hematology data were collected on the 29th day. Results were presented as mean ± standard deviation. One-way analysis of variance (ANOVA) was performed if assumptions were met; otherwise Kruskal-Wallis analysis was performed. RESULT:Oral administration of E. kebericho decoction showed no treatment-related mortality in female rats up to the dose of 5000 mg/kg. In sub-acute toxicity studies, no significant treatment-related abnormalities were observed compared to negative controls. Food consumption, body weight, organ weight, hematology, clinical chemistry, and histopathology did not show significant variation between controls and treatment groups. However, creatinine, relative lung weight, triglycerides, and monocytes were lower in treated compared to control groups. Significant variations between male and female groups in food consumption, relative organ weight, hematology, clinical chemistry were observed. Histolo-pathology of high-dose treated groups showed fatty liver. CONCLUSION:Echinops kebericho showed LD50 of greater than 5000 mg/kg in acute toxicity study and is well tolerated up to the dose of 600 mg/kg body weight in sub-acute toxicity study.

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Acute and repeated-dose toxicity of Echinops kebericho Mesfin essential oil

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