Dataset Information

The effect of high-fat diet and exercise on KISS-1/GPR54 expression in testis of growing rats.

ABSTRACT:

Purpose

To find the expression of KISS-1 and G protein-coupled receptor 54 in rats testis from PND 21st to 56th.

Method

128 three-week-old weaned rats underwent high-fat diet and exercise (60-70% VO_2max, 1 h/day, 5 days/week) intervention and were randomly divided into group C, CE, HC, or HE. Sample time points were set on the PND 21st, 35th, 43rd, and 56th. The testicular testosterone and the mRNA content, and protein content of KISS-1 and GPR54 in testis tissue were detected by ELISA, RT-qPCR, and Western blotting.

Result

(1) The protein of KISS-1 and GPR54 increased gradually during the growing period. KISS-1 mRNA peaked at 35D and GPR54 peaked at 43D. (2) High-fat diet affected the expression of the KISS-1/GPR54 system in rat testis and reduced the expression level of KISS-1 protein. (3) 60-70% VO₂max exercise decreased the KISS-1/GPR54 expression level. Exercise intervention improved testicular development in rats with a high-fat diet.

Conclusion

The expression of KISS-1/GPR54 increased during the growing period. High-fat diet can downregulate the protein and gene expression of KISS-1/GPR54 and change the expression trend. 60-70% VO₂max exercise decreased the expression of KISS-1/GPR54, which may be involved in the effects of exercise on high-fat dietary sex hormone disorders.

SUBMITTER: Feng J

PROVIDER: S-EPMC7788936 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Publications

The effect of high-fat diet and exercise on KISS-1/GPR54 expression in testis of growing rats.

Feng Junpeng J Xu Rui R Li Yafei Y Zhou Qishu Q Song Ge G Deng Yimin Y Yan Yi Y

Nutrition & metabolism 20210106 1

<h4>Purpose</h4>To find the expression of KISS-1 and G protein-coupled receptor 54 in rats testis from PND 21st to 56th.<h4>Method</h4>128 three-week-old weaned rats underwent high-fat diet and exercise (60-70% VO<sub>2max</sub>, 1 h/day, 5 days/week) intervention and were randomly divided into group C, CE, HC, or HE. Sample time points were set on the PND 21st, 35th, 43rd, and 56th. The testicular testosterone and the mRNA content, and protein content of KISS-1 and GPR54 in testis tissue were d ...[more]

PMID: 33407658

Similar Datasets

Project description:BackgroundExcessive gestational weight gain (GWG), which is associated with adverse long-term effects on the health of the offspring, has become a major clinical problem. Accumulating evidence indicates that the ovary kisspeptin/GPR54 system directly participates in a series of physiological activities. We used a model of high-fat diet (HFD) during gestational to investigate offspring's ovarian function and whether kisspeptin/GPR54 system is involved.MethodsAfter introducing the male and confirmation of mating by checking a vaginal sperm plug, female rats were randomized into two groups: control diet called NCD group and high-fat diet called HFD group. After birth, all rats were changed into a control diet and litter size was adjusted to 12 pups per litter. Ovaries were collected for assessment at postnatal day (PND) 4 and PND 30. The timing of vaginal opening was recorded, and the estrous cyclicity was monitored for 2 consecutive weeks immediately. Primary granulosa cells and ovaries which were taken from PND 4 were collected for determination of the direct effect of kisspeptin-10 (kp-10) in vitro.ResultsNeonatal rats exposed to HFD during gestation had a lower number of secondary follicles in the ovary. The expression of follicle-stimulating hormone receptor (FSHR) and kisspeptin was not altered. At prepuberty, the number of antral follicles and preovulatory follicles was elevated with decreased type III follicles in the HFD group. While the expression of ovulation-related genes was decreased, the expression levels of follicular growth-related genes and steroidogenesis synthesis related genes were elevated. A significant increase in kiss1 mRNA and kisspeptin protein was detected without changes in kiss1r mRNA and GPR54. Maternal high-fat diet during gestation resulted in a significant advanced puberty onset and an irregular estrous cycle in offspring rats. In addition, the administration of kp-10 produced an increase in viability of primary granulosa cells and enlarged the size of oocytes.ConclusionsHFD exposure during maternal gestation had a long-term effect on reproductive function in the offspring and the increased ovarian kisspeptin/GPR54 system might be involved.

Dataset Information

The effect of high-fat diet and exercise on KISS-1/GPR54 expression in testis of growing rats.

Purpose

Method

Result

Conclusion

Publications

The effect of high-fat diet and exercise on KISS-1/GPR54 expression in testis of growing rats.

Similar Datasets

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