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ABSTRACT: Background
Polycystic ovary syndrome (PCOS) is a common endocrine disease of the female reproductive system that seriously affects women's health. Berberine (BBR) has many pharmacological properties and is used as an insulin sensitizer. This study aimed to investigate the effect of BBR on PCOS and explore its related mechanisms.Methods
Forty-two rats were randomly divided into the following six groups (n?=?7 per group): control, control + BBR, PCOS-normal diet (ND), PCOS-ND?+?BBR, PCOS-high-fat diet (HFD), and PCOS-HFD?+?BBR. The PCOS rat models were established by injecting rats with dehydroepiandrosterone. Further, the rats were gavaged with BBR (150?mg/kg/d) for 6 weeks. Then, the body weight, HOMA-IR, and testosterone levels of all rats were determined. Cell apoptosis of ovary granulosa cells was determined by a TUNEL assay kit. Real-time quantification PCR (RT-qPCR) and western blotting were utilized to evaluate the expression of TLR4, LYN, PI3K, Akt, NF-kB, TNF-?, IL-1, IL-6, and caspase-3.Results
BBR reduced the levels of insulin resistance and testosterone in PCOS rats. Additionally, the cell apoptosis rate increased significantly in PCOS rats (P?ConclusionsBBR may relieve PCOS pathology and IR values by inhibiting cell apoptosis and by regulating the expression levels of TLR4, LYN, PI3K, Akt, NF-kB, TNF-?, IL-1, IL-6, and caspase-3.
SUBMITTER: Shen HR
PROVIDER: S-EPMC7789273 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Shen Hao-Ran HR Xu Xiao X Li Xue-Lian XL
Reproductive biology and endocrinology : RB&E 20210107 1
<h4>Background</h4>Polycystic ovary syndrome (PCOS) is a common endocrine disease of the female reproductive system that seriously affects women's health. Berberine (BBR) has many pharmacological properties and is used as an insulin sensitizer. This study aimed to investigate the effect of BBR on PCOS and explore its related mechanisms.<h4>Methods</h4>Forty-two rats were randomly divided into the following six groups (n = 7 per group): control, control + BBR, PCOS-normal diet (ND), PCOS-ND + BBR ...[more]