Dataset Information

The tail-tale of stress: an exploratory analysis of cortisol levels in the tail-hair of captive Asian elephants.

ABSTRACT:

Background

Assessment of physiological states by measuring biomarkers, such as cortisol, has significantly contributed to the monitoring of health, welfare and management of animals. Immunoreactive cortisol in hair (hC) has been used widely for deciphering 'stressful' past-events in various wild and captive animals. However, no such studies have been done in long-lived mammals.

Methods

In this first exploratory study in elephants, we assessed (i) tail-hair growth rate (TGR) and (ii) hC levels in tail-hair samples from six captive Asian elephants from two zoos in Japan for comparing hC levels with zoo-keepers' records of distinct biological events over a c.0.5-2.0-year period. Tail-hair samples were cut into segments (based on monthly growth rate), pulverized or minced and a validated cortisol enzyme-immunoassay employed to measure hC levels.

Results

When the hC levels of all individuals were compared with the keepers' records, a posteriori, most of the high hC levels were found to be associated with 'stressful' or distinct behavioural events such as pathological (anaemia, colic infection, skin infection, oral sores), psychosocial (reluctance in entering the enclosure, presence of a calf) and husbandry practice-related (contact trials/ space sharing) conditions, indicating that tail-hair indeed can be a potential 'retrospective' calendar of physiological health of an animal.

Conclusions

Our observations open up the possibility of using the tail-hair as an alternative matrix to reconstruct the physiological history of elephants.

SUBMITTER: Pokharel SS

PROVIDER: S-EPMC7789861 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Publications

The tail-tale of stress: an exploratory analysis of cortisol levels in the tail-hair of captive Asian elephants.

Pokharel Sanjeeta Sharma SS Yoneda Hiroki H Yanagi Moe M Sukumar Raman R Kinoshita Kodzue K

PeerJ 20210104

<h4>Background</h4>Assessment of physiological states by measuring biomarkers, such as cortisol, has significantly contributed to the monitoring of health, welfare and management of animals. Immunoreactive cortisol in hair (hC) has been used widely for deciphering 'stressful' past-events in various wild and captive animals. However, no such studies have been done in long-lived mammals.<h4>Methods</h4>In this first exploratory study in elephants, we assessed (i) tail-hair growth rate (TGR) and (i ...[more]

PMID: 33505782

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Project description:Understanding the factors associated with the long-term stress levels of captive animals is important from the view of animal welfare. In this study, we investigated the effects of relocation in addition to individual and environmental factors related to social management on long-term stress level in group-living captive chimpanzees by examining behaviors and hair cortisol (HC). Specifically, we conducted two studies. The first compared changes in HC levels before and after the relocation of 8 chimpanzees (Study 1) and the second examined the relationship between individual and environmental factors and individual HC levels in 58 chimpanzees living in Kumamoto Sanctuary (KS), Kyoto University (Study 2). We hypothesized that relocation, social situation, sex, and early rearing conditions, would affect the HC levels of captive chimpanzees. We cut arm hair from chimpanzees and extracted and assayed cortisol with an enzyme immunoassay. Aggressive behaviors were recorded ad libitum by keepers using a daily behavior monitoring sheet developed for this study. The results of Study 1 indicate that HC levels increased during the first year after relocation to the new environment and then decreased during the second year. We observed individual differences in reactions to relocation and hypothesized that social factors may mediate these changes. In Study 2, we found that the standardized rate of receiving aggression, rearing history, sex, and group formation had a significant influence on mean HC levels. Relocation status was not a significant factor, but mean HC level was positively correlated with the rate of receiving aggression. Mean HC levels were higher in males than in females, and the association between aggressive interactions and HC levels differed by sex. These results suggest that, although relocation can affect long-term stress level, individuals' experiences of aggression and sex may be more important contributors to long-term stress than relocation alone.

Dataset Information

The tail-tale of stress: an exploratory analysis of cortisol levels in the tail-hair of captive Asian elephants.

Background

Methods

Results

Conclusions

Publications

The tail-tale of stress: an exploratory analysis of cortisol levels in the tail-hair of captive Asian elephants.

Similar Datasets

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