Ontology highlight
ABSTRACT:
SUBMITTER: Ning B
PROVIDER: S-EPMC7793573 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Science advances 20210108 2
Point-of-care COVID-19 assays that are more sensitive than the current RT-PCR (reverse transcription polymerase chain reaction) gold standard assay are needed to improve disease control efforts. We describe the development of a portable, ultrasensitive saliva-based COVID-19 assay with a 15-min sample-to-answer time that does not require RNA isolation or laboratory equipment. This assay uses CRISPR-Cas12a activity to enhance viral amplicon signal, which is stimulated by the laser diode of a smart ...[more]