Optimization of a Method to Detect Autoantigen-Specific T-Cell Responses in Type 1 Diabetes.

ABSTRACT: The development of tolerizing therapies aiming to inactivate autoreactive effector T-cells is a promising therapeutic approach to control undesired autoimmune responses in human diseases such as Type 1 Diabetes (T1D). A critical issue is a lack of sensitive and reproducible methods to analyze antigen-specific T-cell responses, despite various attempts. We refined a proliferation assay using the fluorescent dye 5,6-carboxylfluorescein diacetate succinimidyl ester (CFSE) to detect responding T-cells, highlighting the fundamental issues to be taken into consideration to monitor antigen-specific responses in patients with T1D. The critical elements that maximize detection of antigen-specific responses in T1D are reduction of blood storage time, standardization of gating parameters, titration o

SUBMITTER: Musthaffa Y 

PROVIDER: S-EPMC7793893 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature