Ontology highlight
ABSTRACT:
SUBMITTER: Summers PA
PROVIDER: S-EPMC7794231 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Summers Peter A PA Lewis Benjamin W BW Gonzalez-Garcia Jorge J Porreca Rosa M RM Lim Aaron H M AHM Cadinu Paolo P Martin-Pintado Nerea N Mann David J DJ Edel Joshua B JB Vannier Jean Baptiste JB Kuimova Marina K MK Vilar Ramon R
Nature communications 20210108 1
Guanine rich regions of oligonucleotides fold into quadruple-stranded structures called G-quadruplexes (G4s). Increasing evidence suggests that these G4 structures form in vivo and play a crucial role in cellular processes. However, their direct observation in live cells remains a challenge. Here we demonstrate that a fluorescent probe (DAOTA-M2) in conjunction with fluorescence lifetime imaging microscopy (FLIM) can identify G4s within nuclei of live and fixed cells. We present a FLIM-based cel ...[more]