Ontology highlight
ABSTRACT:
SUBMITTER: Tang L
PROVIDER: S-EPMC7794744 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Tang Longteng L Zhang Shuce S Zhao Yufeng Y Rozanov Nikita D ND Zhu Liangdong L Wu Jiahui J Campbell Robert E RE Fang Chong C
International journal of molecular sciences 20210105 1
Ratiometric indicators with long emission wavelengths are highly preferred in modern bioimaging and life sciences. Herein, we elucidated the working mechanism of a standalone red fluorescent protein (FP)-based Ca<sup>2+</sup> biosensor, REX-GECO1, using a series of spectroscopic and computational methods. Upon 480 nm photoexcitation, the Ca<sup>2+</sup>-free biosensor chromophore becomes trapped in an excited dark state. Binding with Ca<sup>2+</sup> switches the route to ultrafast excited-state ...[more]