Ontology highlight
ABSTRACT:
SUBMITTER: Xue C
PROVIDER: S-EPMC7797033 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Xue Chaoyou C Molnarova Lucia L Steinfeld Justin B JB Zhao Weixing W Ma Chujian C Spirek Mario M Kaniecki Kyle K Kwon Youngho Y Beláň Ondrej O Krejci Katerina K Boulton Simon J SJ Sung Patrick P Greene Eric C EC Krejci Lumir L
Nucleic acids research 20210101 1
RECQ5 is one of five RecQ helicases found in humans and is thought to participate in homologous DNA recombination by acting as a negative regulator of the recombinase protein RAD51. Here, we use kinetic and single molecule imaging methods to monitor RECQ5 behavior on various nucleoprotein complexes. Our data demonstrate that RECQ5 can act as an ATP-dependent single-stranded DNA (ssDNA) motor protein and can translocate on ssDNA that is bound by replication protein A (RPA). RECQ5 can also translo ...[more]