Protein over-expression in Escherichia coli triggers adaptation analogous to antimicrobial resistance.
Ontology highlight
ABSTRACT: Background
The E. coli pET system is the most widely used protein over-expression system worldwide. It relies on the assumption that all cells produce target protein and it is generally believed that integral membrane protein (IMP) over-expression is more toxic than their soluble counterparts.Results
Using GFP-tagged proteins, high level over-expression of either soluble or IMP targets results in?>?99.9% cell loss with survival rate of only?ConclusionsWe demonstrate that it is the act of high-level over-expression of exogenous proteins in E. coli that sets in motion a chain of events leading to?>?99.9% cell death. These results redefine our understanding of protein over-production and link it to the adaptive survival response seen in the development of antimicrobial resistance.
SUBMITTER: James J
PROVIDER: S-EPMC7798265 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Publications
Protein over-expression in Escherichia coli triggers adaptation analogous to antimicrobial resistance.
James Jack J Yarnall Benjamin B Koranteng Andy A Gibson Jane J Rahman Tahmina T Doyle Declan A DA
Microbial cell factories 20210111 1
<h4>Background</h4>The E. coli pET system is the most widely used protein over-expression system worldwide. It relies on the assumption that all cells produce target protein and it is generally believed that integral membrane protein (IMP) over-expression is more toxic than their soluble counterparts.<h4>Results</h4>Using GFP-tagged proteins, high level over-expression of either soluble or IMP targets results in > 99.9% cell loss with survival rate of only < 0.03%. Selective pressure generates t ...[more]
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