Time-resolved cryo-EM using Spotiton.
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ABSTRACT:
SUBMITTER: Dandey VP
PROVIDER: S-EPMC7799389 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Publications
Time-resolved cryo-EM using Spotiton.
Dandey Venkata P VP Budell William C WC Wei Hui H Bobe Daija D Maruthi Kashyap K Kopylov Mykhailo M Eng Edward T ET Kahn Peter A PA Hinshaw Jenny E JE Kundu Nidhi N Nimigean Crina M CM Fan Chen C Sukomon Nattakan N Darst Seth A SA Saecker Ruth M RM Chen James J Malone Brandon B Potter Clinton S CS Carragher Bridget B
Nature methods 20200810 9
We present an approach for preparing cryo-electron microscopy (cryo-EM) grids to study short-lived molecular states. Using piezoelectric dispensing, two independent streams of ~50-pl droplets of sample are deposited within 10 ms of each other onto the surface of a nanowire EM grid, and the mixing reaction stops when the grid is vitrified in liquid ethane ~100 ms later. We demonstrate this approach for four biological systems where short-lived states are of high interest. ...[more]
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