Development of SYBR Green I-based polymerase chain reaction for feline bocavirus 1 detection.

ABSTRACT: Feline bocavirus 1 (FBoV-1) may be associated with diarrhea in cats. In this study, a SYBR Green I-based quantitative polymerase chain reaction (qPCR) assay was established to detect FBoV-1. The melting curve showed a single melting peak at 83.0 ℃. The results of sensitivity showed that the detection limit of the qPCR was 3.87 × 10¹ copies/μL. Of note, the detection limit of the conventional polymerase chain reaction (cPCR) was 3.87 × 10³ copies/μL. The highest intra-assay and inter-assay coefficients of variation (CV%) were 0.98% and 1.42%, respectively. The positive detection rate of 128 clinical samples using the qPCR and the cPCR was 7.0% (9/128) and 4.7% (6/128), respectively. Taken together, these results indicated that the established qPCR assay has good sensit

SUBMITTER: Wang Y 

PROVIDER: S-EPMC7799429 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature