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Sequencing complete mitochondrial genome of Lanius sphenocercus sphenocercus (Passeriformes: Laniidae) using Illunima HiSeq 2500.

ABSTRACT: Using an Illumina platform, we sequenced the complete mitochondrial genome of Lanius sphenocercus sphenocercus to an average coverage of 1669.5×. We performed a de novo assembly using SOAPdenovo2 and obtained the total mitogenome with 16,833?bp in length. Most PCGs begin with the typical ATG start codon with the exception of COI gene, which use GTG as the initiation codon. Stop codons AGG, TAG, TAA, and AGA are present in the PCGs; exceptions are COII, COIII and ND4, which possess incomplete termination codons (T). But, the function of COII with incomplete stop codon T should be further investigated. The phylogeny revealed that genetic distance of Laniidae and Corvidae was closer than other species. Compared to other three shrike species, L. s. sphenocercus occupy a separate status in the genus Lanius.

SUBMITTER: Yang MX

PROVIDER: S-EPMC7799910 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

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Sequencing complete mitochondrial genome of Lanius sphenocercus sphenocercus (Passeriformes: Laniidae) using Illunima HiSeq 2500.

Yang Mei-Xia MX Wang Qing-Xiong QX Xiao Hong H Yang Chao C

Mitochondrial DNA. Part B, Resources 20160419 1

Using an Illumina platform, we sequenced the complete mitochondrial genome of Lanius sphenocercus sphenocercus to an average coverage of 1669.5×. We performed a de novo assembly using SOAPdenovo2 and obtained the total mitogenome with 16,833 bp in length. Most PCGs begin with the typical ATG start codon with the exception of COI gene, which use GTG as the initiation codon. Stop codons AGG, TAG, TAA, and AGA are present in the PCGs; exceptions are COII, COIII and ND ...[more]

PMID: 33473475

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