Dataset Information

The complete mitogenome of the Galaxy Coral, Galaxea fascicularis (Cnidaria: Oculinidae).

ABSTRACT: In this study, the complete mitogenome sequence of Galaxy Coral, Galaxea fascicularis (Cnidaria: Oculinidae), has been sequenced by next-generation sequencing method. The overall base composition of Galaxea fascicularis mitogenome is 24.9% for A, 14.0% for C, 24.3% for G and 36.8% for T and has low GC content of 38.3%. The assembled mitogenome, consisting of 18 751 bp, has unique 13 protein-coding genes (PCGs), three transfer RNAs and two ribosomal RNAs genes. The G. fascicularis mitogenome has the common mitogenome gene organization and feature of scleractinian coral. Among 13 PCGs, ND5 gene is interrupted by group I intron (12?022?bp). There are 12 genes embedded in ND5 group I intron (tRNA-Leu, ND1, CYTB, ND2, ND6, ATP6, ND4, 12S rRNA, COX3, COX2, ND4L and ND3). The complete mitogenome provides essential and important DNA molecular data for further phylogenetic and evolutionary analysis for stony coral.

SUBMITTER: Niu W

PROVIDER: S-EPMC7800161 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

The complete mitogenome of the Galaxy Coral, Galaxea fascicularis (Cnidaria: Oculinidae).

Niu Wentao W Huang Hui H Lin Rongcheng R Chen Ching-Hung CH Shen Kang-Ning KN Hsiao Chung-Der CD

Mitochondrial DNA. Part B, Resources 20160201 1

In this study, the complete mitogenome sequence of Galaxy Coral, Galaxea fascicularis (Cnidaria: Oculinidae), has been sequenced by next-generation sequencing method. The overall base composition of Galaxea fascicularis mitogenome is 24.9% for A, 14.0% for C, 24.3% for G and 36.8% for T and has low GC content of 38.3%. The assembled mitogenome, consisting of 18 751 bp, has unique 13 protein-coding genes (PCGs), three transfer RNAs and two ribosomal RNAs genes. The G. fasciculari ...[more]

PMID: 33473388

Similar Datasets

Project description:The symbiosis between cnidarian hosts and photosynthetic dinoflagellates of the family Symbiodiniaceae (i.e., zooxanthellae) provides the energy foundation of coral reef ecosystems in oligotrophic waters. The structure of symbiont biota and the dominant species of algal symbiont partly shape the environmental adaptability of coral symbiotes. In this study, the algal symbiont cells were isolated from the tentacles of Galaxea fascicularis, a hermatypic coral with obvious differentiation in heat resistance, and were cultured in vitro with an improved L1 medium. An algal monoclonal cell line was established using separated algal culture drops and soft agar plating method, and named by GF19C1 as it was identified as Cladocopium sp. C1 (Symbiodiniaceae) based on its ITS1, ITS2, and the non-coding region of the plastid psbA minicircle (psbA ncr ) sequences. Most GF19C1 cells were at the coccoid stage of the gymnodinioid, their markedly thickened (ca. two times) cell wall suggests that they developed into vegetative cysts and have sexual and asexual reproductive potential. The average diameter of GF19C1 cells decreased significantly, probably due to the increasing mitotic rate. The chloroplasts volume density of GF19C1 was significantly lower than that of their symbiotic congeners, while the surface area density of thylakoids relative to volumes of chloroplasts was not significantly changed. The volume fraction of vacuoles increased by nearly fivefold, but there was no significant change in mitochondria and accumulation bodies. Light-temperature orthogonal experiments showed that, GF19C1 growth preferred the temperature 25 ± 1°C (at which it is maintained post-isolation) rather than 28 ± 1°C under the light intensity of 42 ± 2 or 62 ± 2 ?mol photons m-2 s-1, indicating an inertia for temperature adaptation. The optimum salinity for GF19C1 growth ranged between 28-32 ppt. The monoclonal culture techniques established in this study were critical to clarify the physiological and ecological characteristics of various algal symbiont species, and will be instrumental to further reveal the roles of algal symbionts in the adaptive differentiation of coral-zooxanthellae holobionts in future studies.

Dataset Information

The complete mitogenome of the Galaxy Coral, Galaxea fascicularis (Cnidaria: Oculinidae).

Publications

The complete mitogenome of the Galaxy Coral, <i>Galaxea fascicularis</i> (Cnidaria: Oculinidae).

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets