Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The complete mitochondrial genome of the tea lace bug, Stephanitis chinensis (Hemiptera: Tingidae).

ABSTRACT: The tea lace bug, Stephanitis chinensis Drake (Hemiptera: Tingidae), is a pest which feeds on the undersides of tea leaves by piercing the epidermis and sucking the sap, and causes great harm to plant growth and tea production. We have obtained the whole mitochondrial genome of S. chinensis (GenBank accession No. MF498769). The entire mt genome is 16,667?bp in size with an A?+?T content of 78.41%. The tea lace bug mt genome encodes all 37 genes that are typically found in animal mt genomes, consists of 13 protein-coding genes (PCGs), 2 ribosomal RNA, and 22 transfer RNA genes. The gene order is consistent with other sequenced mt genome of lace bugs. The A?+?T-rich region of this genome is 2215?bp long with the A?+?T content of 82.58%, and located between the rrnS and trnI genes. Phylogenetic analysis performed using 13 PCGs with 14 heteropteran insects showed that S. chinensis clusters with other Tingidae species.

SUBMITTER: Li PW

PROVIDER: S-EPMC7800372 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

The complete mitochondrial genome of the tea lace bug, Stephanitis chinensis (Hemiptera: Tingidae).

Li Pin-Wu PW Wang Xiao-Qing XQ Chen Shi-Chun SC Peng Ping P

Mitochondrial DNA. Part B, Resources 20170906 2

The tea lace bug, Stephanitis chinensis Drake (Hemiptera: Tingidae), is a pest which feeds on the undersides of tea leaves by piercing the epidermis and sucking the sap, and causes great harm to plant growth and tea production. We have obtained the whole mitochondrial genome of S. chinensis (GenBank accession No. MF498769). The entire mt genome is 16,667 bp in size with an A + T content of 78.41%. The tea lace bug mt genome encodes all 37 genes that are typically found in animal mt ...[more]

PMID: 33490467

Similar Datasets

Sequencing and Characterization of the Invasive Sycamore Lace Bug Corythucha ciliata (Hemiptera: Tingidae) Transcriptome.

Project description:The sycamore lace bug, Corythucha ciliata (Hemiptera: Tingidae), is an invasive forestry pest rapidly expanding in many countries. This pest poses a considerable threat to the urban forestry ecosystem, especially to Platanus spp. However, its molecular biology and biochemistry are poorly understood. This study reports the first C. ciliata transcriptome, encompassing three different life stages (Nymphs, adults female (AF) and adults male (AM)). In total, 26.53 GB of clean data and 60,879 unigenes were obtained from three RNA-seq libraries. These unigenes were annotated and classified by Nr (NCBI non-redundant protein sequences), Nt (NCBI non-redundant nucleotide sequences), Pfam (Protein family), KOG/COG (Clusters of Orthologous Groups of proteins), Swiss-Prot (A manually annotated and reviewed protein sequence database), and KO (KEGG Ortholog database). After all pairwise comparisons between these three different samples, a large number of differentially expressed genes were revealed. The dramatic differences in global gene expression profiles were found between distinct life stages (nymphs and AF, nymphs and AM) and sex difference (AF and AM), with some of the significantly differentially expressed genes (DEGs) being related to metamorphosis, digestion, immune and sex difference. The different express of unigenes were validated through quantitative Real-Time PCR (qRT-PCR) for 16 randomly selected unigenes. In addition, 17,462 potential simple sequence repeat molecular markers were identified in these transcriptome resources. These comprehensive C. ciliata transcriptomic information can be utilized to promote the development of environmentally friendly methodologies to disrupt the processes of metamorphosis, digestion, immune and sex differences.

| S-EPMC4975459 | biostudies-literature

Identification of a sex pheromone of the chrysanthemum lace bug Corythucha marmorata (Hemiptera: Tingidae).

Project description:Although the nymphs of Corythucha marmorata form clusters on the undersides of host plant leaves, as frequently observed for Hemiptera, the adults are scattered in the vicinity of the nymph population. By investigating the biological activities of volatile secretions from the adult, we found that the secretions activated male mounting behaviour. A chemical analysis revealed that borneol was a common component of the secretions from both sexes. The absolute configuration of the natural product was the (+)-enantiomer of borneol and the optical isomer was undetectable. Although (+)-borneol showed significant sex pheromone activity against males, the antipode (-)-borneol also induced sex pheromone activity, albeit only slightly. Males may not have a strict identification mechanism based on stereochemistry. To verify the origin of this sex pheromone, we analysed the components of the essential oil of the leaves of Solidago canadensis L. (Compositae: Asteraceae), a host plant; bornyl acetate was detected to be a major component. The plant-produced bornyl acetate had different stereochemistry from the sex pheromone. The results suggested that the adults do not utilise the secondary metabolites of plants but biosynthesise this sex pheromone themselves. This is the first report on sex pheromone identification in Tingidae.

| S-EPMC5544738 | biostudies-other

Attracting Chrysopidae With Plant Volatiles for Lace Bug (Hemiptera: Tingidae) Control in Rhododendrons and Azaleas.

Project description:The azalea lace bug (Stephanitis pyrioides Scott) (Hemiptera: Tingidae) is an invasive pest of rhododendrons and azaleas (Ericaceae: Rhododendron), which feeds on the underside of leaves causing chlorosis, reduced photosynthesis, and even plant death. While insecticides can control this pest, growers, landscape managers, and homeowners have requested softer alternatives. Augmentative release of predatory green lacewing Chrysoperla sp. (Neuroptera: Chrysopidae) eggs and larvae has reduced S. pyrioides, but large-scale implementation may not be practical nor cost-effective. Attracting naturally occurring Chrysopidae with plant volatiles may be an economical and convenient option. In this study, we tested whether volatile blends 1) attracted Chrysoperla sp., and 2) controlled S. pyrioides populations on Rhododendron spp. in farm or urban landscapes. Experimental plots contained different multicomponent lures placed aboveground next to infested plants. Adult Chrysoperla sp., other natural enemies, and S. pyrioides from egg to adult stages were monitored in both farm and urban landscapes for two summers. Overall, two out of three volatile blends consistently attracted Chrysoperla sp. to sticky traps near baited plants. Methyl salicylate + acetic acid + 2-phenylethanol (methyl salicylate blend) and acetophenone + acetic acid + 2-phenylethanol (acetophenone blend) captured more adult Chrysoperla sp. than control traps in farm landscapes. However, only the acetophenone blend was associated with a slight reduction of S. pyrioides. Additional research is needed to determine whether the phenology of the first generation of both species are synchronized for effective season biological control in the Pacific Northwest.

| S-EPMC7459699 | biostudies-literature

Variability of 18rDNA loci in four lace bug species (Hemiptera, Tingidae) with the same chromosome number.

Project description:Male karyotypes of Elasmotropis testacea (Herrich-Schaeffer, 1835), Tingis cardui (Linnaeus, 1758), Tingis crispata (Herrich-Schaeffer, 1838), and Agramma femorale Thomson, 1871 (Heteroptera, Cimicomorpha, Tingidae) were analyzed using conventional chromosome staining and FISH with 18S rDNA and (TTAGG) n telomeric probes. The FISH technique was applied for the first time in the Tingidae. In spite of the fact that all species showed the same chromosome number (2n = 12 + XY), they have significant differences in the number and position of rDNA loci. FISH with the classical insect (TTAGG) n probe produced no signals on chromosomes suggesting telomeres in lace bugs to be of some other molecular composition. Tingidae share absence of the (TTAGG) n telomeric sequence with all so far studied taxa of the advanced true bug infraorders Cimicomorpha and Pentatomomorpha.

| S-EPMC4698567 | biostudies-other

New record of the lace bug species Acalypta marginata (Wolff) (Hemiptera: Heteroptera: Tingidae) from Japan.

Project description:BackgroundThe lace bug species Acalypta marginata (Wolff, 1804) has, to date, been widely known to occur in the Palaearctic Region, but has not been recorded from Japan.New informationAcalypta marginata is recorded from Japan for the first time. Its habitat in Japan is the grassland of Hokkaido. A key to the species of Acalypta occurring in Japan is provided.

| S-EPMC7933090 | biostudies-literature

The complete mitochondrial genome of Corythucha marmorata (Hemiptera: Tingidae).

Project description:The complete mitochondrial genome (mitogenome) of Corythucha marmorata has been sequenced and annotated. The entire mitogenome is a typical circle double-stranded DNA molecule of 15,635b p and consisted of 37 genes and a control region in the typical invertebrate mitochondrial gene arrangement. Phylogenetic analysis recovered the momophyly of Gerromorpha, Enicocephalomorpha and Cimicomorpha.

| S-EPMC7799590 | biostudies-literature

Identification of Entomopathogenic Fungi as Naturally Occurring Enemies of the Invasive Oak Lace Bug, Corythucha arcuata (Say) (Hemiptera: Tingidae).

Project description:The oak lace bug (OLB), Corythucha arcuata (Hemiptera: Tingidae), was first identified as an invasive pest in Europe in northern Italy in 2000 and since then it has spread rapidly, attacking large forested areas in European countries. The OLB is a cell sap-sucking insect that is native to North America, with Quercus spp. as its main host. Its rapid expansion, successful establishment in invaded countries, and observations of more damage to hosts compared to native areas are most likely due to a lack of natural enemies, pathogens and competitors. In its native area, various natural enemies of OLBs have been identified; however, little is known about the occurrence and impact of OLB pathogens. None of the pathogenic fungi found on OLBs in natural conditions have been identified until now. In this study, we provide evidence of four entomopathogenic fungi that are naturally occurring on invasive OLBs found in infested pedunculate oak forests in eastern Croatia. On the basis of their morphology and multilocus molecular phylogeny, the fungi were identified as Beauveria pseudobassiana, Lecanicillium pissodis, Akanthomyces attenuatus and Samsoniella alboaurantium. The sequences generated for this study are available from GenBank under the accession numbers MT004817-MT004820, MT004833-MT004835, MT027501-MT27510, and MT001936-MT0011943. These pathogenic species could facilitate biological control strategies against OLBs.

| S-EPMC7600444 | biostudies-literature

First record of the lace bug genus Eritingis (Drake and Ruhoff) (Hemiptera: Heteroptera: Tingidae) from Japan and Thailand.

Project description:BackgroundThe lace bug genus Eritingis Drake and Ruhoff, 1962 is widely distributed in the Australian and Oriental Regions, whereas only a single species, E. recentis (Drake and Poor, 1937), has been recorded from the Oriental Region. To date, E. recentis is known to occur in Indonesia, Malaysia, Papua New Guinea, Singapore and Vietnam, but has not been recorded from Japan and Thailand.New informationEritingis and E. recentis are recorded from Japan and Thailand for the first time.

| S-EPMC8096800 | biostudies-literature

The complete mitochondrial genome of the damsel bug Alloeorhynchus bakeri (Hemiptera: Nabidae).

Project description:The complete sequence of the mitochondrial DNA (mtDNA) of the damsel bug, Alloeorhynchus bakeri, has been completed and annotated in this study. It represents the first sequenced mitochondrial genome of heteropteran family Nabidae. The circular genome is 15, 851 bp in length with an A+T content of 73.5%, contains the typical 37 genes that are arranged in the same order as that of the putative ancestor of hexapods. Nucleotide composition and codon usage are similar to other known heteropteran mitochondrial genomes. All protein-coding genes (PCGs) use standard initiation codons (methionine and isoleucine), except COI, which started with TTG. Canonical TAA and TAG termination codons are found in eight protein-coding genes, the remaining five (COI, COII, COIII, ND5, ND1) have incomplete termination codons (T or TA). PCGs of two strands present opposite CG skew which is also reflected by the nucleotide composition and codon usage. All tRNAs have the typical clover-leaf structure, except the dihydrouridine (DHU) arm of tRNA(Ser (AGN))which forms a simple loop as known in many other metazoa. Secondary structure models of the ribosomal RNA genes of A. bakeri are presented, similar to those proposed for other insect orders. There are six domains and 45 helices and three domains and 27 helices in the secondary structures of rrnL and rrnS, respectively. The major non-coding region (also called control region) between the small ribosomal subunit and the tRNA(Ile )gene includes two special regions. The first region includes four 133 bp tandem repeat units plus a partial copy of the repeat (28 bp of the beginning), and the second region at the end of control region contains 4 potential stem-loop structures. Finally, PCGs sequences were used to perform a phylogenetic study. Both maximum likelihood and Bayesian inference analyses highly support Nabidae as the sister group to Anthocoridae and Miridae.

| S-EPMC3248651 | biostudies-literature

The Complete Mitochondrial Genome of the Plant Bug Lygus pratensis Linnaeus (Hemiptera: Miridae).

Project description:Lygus pratensis is a phytophagous pest responsible for yield losses in Bt alfalfa and other economic crops in Northwestern China. To better characterize Miridae at the genomic level, the complete mitochondrial (mt) genome of L. pratensis was sequenced and analyzed in this study. The mt genome was amplified via the polymerase chain reaction to generate overlapping fragments. These fragments were then sequenced, spliced, and analyzed to include the examination of nucleotide composition, codon usage, compositional biases, protein-coding genes (PCGs), and RNA secondary structures. Phylogenetic relationships between L. pratensis and other species in different Heteroptera families were also examined. The mt genome was found to be a typical circular genome with a length of 16,591 bp and a total AT content of 75.1%, encoded for 13 PCGs, 22 transfer RNAs (tRNAs), 2 ribosomal RNAs (lrRNA and srRNA), and a noncoding control region. The nucleotide composition of the entire mt genome was heavily biased toward A and T. All of the tRNAs were predicted to have classic clover leaf structures, but three of the tRNAs (tRNAAsn, tRNAHis, tRNAHis) were missing the T?C loop. The control region (2,017 bp), which was found to be located between 12S and tRNAIle, contained three tandem repeat elements. Phylogenetic analyses showed that L. pratensis is closely related to the other three examined Lygus bugs, and that it is a sister group to Apolygus and Adelphocoris. This study confirms the usability of the mt genome in phylogenesis studies pertaining to the Lygus genus, within Miridae.

| S-EPMC5893962 | biostudies-literature