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The complete mitochondrial genome and phylogenetic analysis of Haemaphysalis longicornis Neumann (Acari: Ixodidae).

ABSTRACT: Haemaphysalis longicornis ticks are vectors or reservoirs of numerous infectious pathogens and cause a variety of human and animal diseases worldwide. However, there is limited knowledge on available genetic sequence. Herein, we extracted the complete mitochondrial genome (mitogenome) from enriched mitochondria of H. longicornis first time in ticks and gained its sequence with 14,718bp in length. The mitogenome consisted of 13 PCGs, 22 tRNA, 2 rRNA, and 2 noncoding regions. Also, the monophyletic phylogenetic position of H. longicornis is inferred based on 28 complete mitogenomes in total comprised of various species from Ixodida ticks in addition to the mitogenome of H. longicornis.

SUBMITTER: Geng J

PROVIDER: S-EPMC7800405 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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The complete mitochondrial genome and phylogenetic analysis of Haemaphysalis longicornis Neumann (Acari: Ixodidae).

Geng Jingjing J Zheng Aihua A Zou Zhen Z Zhang Xing X

Mitochondrial DNA. Part B, Resources 20171125 2

Haemaphysalis longicornis ticks are vectors or reservoirs of numerous infectious pathogens and cause a variety of human and animal diseases worldwide. However, there is limited knowledge on available genetic sequence. Herein, we extracted the complete mitochondrial genome (mitogenome) from enriched mitochondria of H. longicornis first time in ticks and gained its sequence with 14,718bp in length. The mitogenome consisted of 13 PCGs, 22 tRNA, 2 rRNA, and 2 noncoding regions. Also, t ...[more]

PMID: 33490479

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Project description:BACKGROUND:Close relationships between ticks and microbial communities are important for tick fitness and pathogen colonization and transmission. Haemaphysalis longicornis, distributed widely in China, can carry and transmit various pathogens and pose serious damages to public health and economics. However, little is known about the broader array of microbial communities and symbionts in H. longicornis under natural conditions. In the present study, we investigated the composition of bacterial communities associated with H. longicornis and evaluated the putative symbionts. METHODS:The eubacterial 16S rRNA gene clone libraries of H. longicornis were constructed and analyzed by restriction fragment length polymorphism (RFLP) and DNA sequencing. In addition, diagnostic PCR was performed to assess the prevalence, vertical transmission and infection sites of the symbionts in H. longicornis. RESULTS:Vertically-transmitted symbionts, potential pathogens and allochthonous nonpathogenic bacteria were identified from the field-collected H. longicornis. Three types of symbionts (Coxiella-like, Arsenophonus-like and Rickettsia-like symbionts) were identified in a single host simultaneously. A series of analyses revealed the vertical transmission, prevalence, and infection sites of these symbionts. However, only Coxiella-like bacteria were transmitted stably in the laboratory-reared ticks. In addition, we identified a novel Coxiella-like agent with 95.31% sequence similarity to the taxon described previously. CONCLUSIONS:The present study demonstrated that natural H. longicornis harboured a diverse array of microbial communities. Three types of symbionts were identified in a single host simultaneously. Moreover, high prevalence, vertical transmission and the infection sites supported an obligate symbiotic association between Coxiella symbiont and its host. The role of Coxiella symbiont in the host fitness and the interaction among microbial communities remained to be elucidated. Our investigation of microbial communities in the ticks revealed the complexity of ecological interactions between host and microbe and provided insight for the biological control of ticks.

Dataset Information

The complete mitochondrial genome and phylogenetic analysis of Haemaphysalis longicornis Neumann (Acari: Ixodidae).

Publications

The complete mitochondrial genome and phylogenetic analysis of <i>Haemaphysalis longicornis</i> Neumann (Acari: Ixodidae).

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