Dataset Information

Characterization of the complete chloroplast genome of Musella lasiocarpa.

ABSTRACT: The whole chloroplast (cp) genome sequence of Musella lasiocarpa has been characterized from Illumina pair-end sequencing. The complete cp genome was 169,178?bp in length, containing a large single copy (LSC) region of 87,884?bp and a small single copy (SSC) region of 11,144?bp, which were separated by a pair of 35,075?bp inverted repeat (IR) regions. The genome contained 138 genes, including 88 protein-coding genes (87 PCG species), 37 tRNA genes (30 tRNA species), and eight ribosomal RNA genes (four rRNA species). The most of gene species occur as a single copy, while 23 gene species occur in double copies. The overall AT content of M. lasiocarpa cp genome is 63.3%, while the corresponding values of the LSC, SSC, and IR regions are 64.9, 69.2, and 60.3%, respectively. The cp genome sequence is similar to that of the genus Musa.

SUBMITTER: Zhang L

PROVIDER: S-EPMC7800482 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Characterization of the complete chloroplast genome of <i>Musella lasiocarpa</i>.

Zhang Lei L Guo Xinyi X Wang Zefu Z Wang Mingcheng M Hu Quanjun Q

Mitochondrial DNA. Part B, Resources 20180705 2

The whole chloroplast (cp) genome sequence of <i>Musella lasiocarpa</i> has been characterized from Illumina pair-end sequencing. The complete cp genome was 169,178 bp in length, containing a large single copy (LSC) region of 87,884 bp and a small single copy (SSC) region of 11,144 bp, which were separated by a pair of 35,075 bp inverted repeat (IR) regions. The genome contained 138 genes, including 88 protein-coding genes (87 PCG species), 37 tRNA genes (30 tRNA species), and eight ribosomal RN ...[more]

PMID: 33474300

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Project description:Erigeron breviscapus is a famous medicinal plant. However, the limited chloroplast genome information of E. breviscapus, especially for the chloroplast DNA sequence resources, has hindered the study of E. breviscapus chloroplast genome transformation. Here, the complete chloroplast (cp) genome of E. breviscapus was reported. This genome was 152,164bp in length, included 37.2% GC content and was structurally arranged into two 24,699bp inverted repeats (IRs) and two single-copy areas. The sizes of the large single-copy region and the small single-copy region were 84,657 and 18,109bp, respectively. The E. breviscapus cp genome consisted of 127 coding genes, including 83 protein coding genes, 36 transfer RNA (tRNA) genes, and eight ribosomal RNA (rRNA) genes. For those genes, 95 genes were single copy genes and 16 genes were duplicated in two inverted regions with seven tRNAs, four rRNAs, and five protein coding genes. Then, genomic DNA of E. breviscapus was used as a template, and the endogenous 5' and 3' flanking sequences of the trnI gene and trnA gene were selected as homologous recombinant fragments in vector construction and cloned through PCR. The endogenous 5' flanking sequences of the psbA gene and rrn16S gene, the endogenous 3' flanking sequences of the psbA gene, rbcL gene, and rps16 gene and one sequence element from the psbN-psbH chloroplast operon were cloned, and certain chloroplast regulatory elements were identified. Two homologous recombination fragments and all of these elements were constructed into the cloning vector pBluescript SK (+) to yield a series of chloroplast expression vectors, which harbored the reporter gene EGFP and the selectable marker aadA gene. After identification, the chloroplast expression vectors were transformed into Escherichia coli and the function of predicted regulatory elements was confirmed by a spectinomycin resistance test and fluorescence intensity measurement. The results indicated that aadA gene and EGFP gene were efficiently expressed under the regulation of predicted regulatory elements and the chloroplast expression vector had been successfully constructed, thereby providing a solid foundation for establishing subsequent E. breviscapus chloroplast transformation system and genetic improvement of E. breviscapus.

Dataset Information

Characterization of the complete chloroplast genome of Musella lasiocarpa.

Publications

Characterization of the complete chloroplast genome of <i>Musella lasiocarpa</i>.

Similar Datasets

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