Unknown

Dataset Information

0

Duration and key determinants of infectious virus shedding in hospitalized patients with coronavirus disease-2019 (COVID-19).

ABSTRACT: Key questions in COVID-19 are the duration and determinants of infectious virus shedding. Here, we report that infectious virus shedding is detected by virus cultures in 23 of the 129 patients (17.8%) hospitalized with COVID-19. The median duration of shedding infectious virus is 8 days post onset of symptoms (IQR 5-11) and drops below 5% after 15.2 days post onset of symptoms (95% confidence interval (CI) 13.4-17.2). Multivariate analyses identify viral loads above 7 log10 RNA copies/mL (odds ratio [OR] of 14.7 (CI 3.57-58.1; p < 0.001) as independently associated with isolation of infectious SARS-CoV-2 from the respiratory tract. A serum neutralizing antibody titre of at least 1:20 (OR of 0.01 (CI 0.003-0.08; p < 0.001) is independently associated with non-infectious SARS-CoV-2. We conclude that quantitative viral RNA load assays and serological assays could be used in test-based strategies to discontinue or de-escalate infection prevention and control precautions.

SUBMITTER: van Kampen JJA 

PROVIDER: S-EPMC7801729 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Duration and key determinants of infectious virus shedding in hospitalized patients with coronavirus disease-2019 (COVID-19).

van Kampen Jeroen J A JJA   van de Vijver David A M C DAMC   Fraaij Pieter L A PLA   Haagmans Bart L BL   Lamers Mart M MM   Okba Nisreen N   van den Akker Johannes P C JPC   Endeman Henrik H   Gommers Diederik A M P J DAMPJ   Cornelissen Jan J JJ   Hoek Rogier A S RAS   van der Eerden Menno M MM   Hesselink Dennis A DA   Metselaar Herold J HJ   Verbon Annelies A   de Steenwinkel Jurriaan E M JEM   Aron Georgina I GI   van Gorp Eric C M ECM   van Boheemen Sander S   Voermans Jolanda C JC   Boucher Charles A B CAB   Molenkamp Richard R   Koopmans Marion P G MPG   Geurtsvankessel Corine C   van der Eijk Annemiek A AA  

Nature communications 20210111 1

Key questions in COVID-19 are the duration and determinants of infectious virus shedding. Here, we report that infectious virus shedding is detected by virus cultures in 23 of the 129 patients (17.8%) hospitalized with COVID-19. The median duration of shedding infectious virus is 8 days post onset of symptoms (IQR 5-11) and drops below 5% after 15.2 days post onset of symptoms (95% confidence interval (CI) 13.4-17.2). Multivariate analyses identify viral loads above 7 log<sub>10</sub> RNA copies  ...[more]