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Interleukin-6 promotes primitive endoderm development in bovine blastocysts.

ABSTRACT:

Background

Interleukin-6 (IL6) was recently identified as an embryotrophic factor in bovine embryos, where it acts primarily to mediate inner cell mass (ICM) size. This work explored whether IL6 affects epiblast (EPI) and primitive endoderm (PE) development, the two embryonic lineages generated from the ICM after its formation. Nuclear markers for EPI (NANOG) and PE (GATA6) were used to differentiate the two cell types.

Results

Increases (P?ConclusionsTo conclude, IL6 manipulates ICM development after EPI/PE cell fates are established. The PE cells are the target for IL6, where a JAK-dependent signal is used to regulate PE numbers.

SUBMITTER: Wooldridge LK

PROVIDER: S-EPMC7802221 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Interleukin-6 promotes primitive endoderm development in bovine blastocysts.

Wooldridge Lydia K LK Ealy Alan D AD

BMC developmental biology 20210112 1

<h4>Background</h4>Interleukin-6 (IL6) was recently identified as an embryotrophic factor in bovine embryos, where it acts primarily to mediate inner cell mass (ICM) size. This work explored whether IL6 affects epiblast (EPI) and primitive endoderm (PE) development, the two embryonic lineages generated from the ICM after its formation. Nuclear markers for EPI (NANOG) and PE (GATA6) were used to differentiate the two cell types.<h4>Results</h4>Increases (P < 0.05) in total ICM cell numbers and PE ...[more]

PMID: 33430761

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