Ontology highlight
ABSTRACT:
SUBMITTER: Xin R
PROVIDER: S-EPMC7803736 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Xin Ruijiao R Gao Yan Y Gao Yuan Y Wang Robert R Kadash-Edmondson Kathryn E KE Liu Bo B Wang Yadong Y Lin Lan L Xing Yi Y
Nature communications 20210112 1
Circular RNAs (circRNAs) have emerged as an important class of functional RNA molecules. Short-read RNA sequencing (RNA-seq) is a widely used strategy to identify circRNAs. However, an inherent limitation of short-read RNA-seq is that it does not experimentally determine the full-length sequences and exact exonic compositions of circRNAs. Here, we report isoCirc, a strategy for sequencing full-length circRNA isoforms, using rolling circle amplification followed by nanopore long-read sequencing. ...[more]