Ontology highlight
ABSTRACT:
SUBMITTER: Doma E
PROVIDER: S-EPMC7805318 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Doma Eszter E Mayer Isabella Maria IM Brandstoetter Tania T Maurer Barbara B Grausenburger Reinhard R Menzl Ingeborg I Zojer Markus M Hoelbl-Kovacic Andrea A Carlsson Leif L Heller Gerwin G Kollmann Karoline K Sexl Veronika V
Blood advances 20210101 1
Studies of molecular mechanisms of hematopoiesis and leukemogenesis are hampered by the unavailability of progenitor cell lines that accurately mimic the situation in vivo. We now report a robust method to generate and maintain LSK (Lin-, Sca-1+, c-Kit+) cells, which closely resemble MPP1 cells. HPCLSKs reconstitute hematopoiesis in lethally irradiated recipient mice over >8 months. Upon transformation with different oncogenes including BCR/ABL, FLT3-ITD, or MLL-AF9, their leukemic counterparts ...[more]