Injury measurements improve interpretation of thrombus formation data in the cremaster arteriole laser-induced injury model of thrombosis.

ABSTRACT:

Background

The cremaster arteriole laser-induced injury model is a powerful technique with which to investigate the molecular mechanisms that drive thrombus formation. This model is capable of direct visualization and quantification of accumulation of thrombus constituents, including both platelets and fibrin. However, a large degree of variability in platelet accumulation and fibrin formation is observed between thrombi. Strategies to understand this variability will enhance performance and standardization of the model. We determined whether ablation injury size contributes to variation in platelet accumulation and fibrin formation and, if so, whether incorporating ablation injury size into measurements reduces variation.

Methods

Thrombus formation was initiated by laser-in

SUBMITTER: Grover SP 

PROVIDER: S-EPMC7805486 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature