Ontology highlight
ABSTRACT:
SUBMITTER: Usaj M
PROVIDER: S-EPMC7806905 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Ušaj Marko M Moretto Luisa L Vemula Venukumar V Salhotra Aseem A Månsson Alf A
Communications biology 20210113 1
Benefits of single molecule studies of biomolecules include the need for minimal amounts of material and the potential to reveal phenomena hidden in ensembles. However, results from recent single molecule studies of fluorescent ATP turnover by myosin are difficult to reconcile with ensemble studies. We found that key reasons are complexities due to dye photophysics and fluorescent contaminants. After eliminating these, through surface cleaning and use of triple state quenchers and redox agents, ...[more]