Direct visualization of virus removal process in hollow fiber membrane using an optical microscope.

ABSTRACT: Virus removal filters developed for the decontamination of small viruses from biotherapeutic products are widely used in basic research and critical step for drug production due to their long-established quality and robust performance. A variety of imaging techniques have been employed to elucidate the mechanism(s) by which viruses are effectively captured by filter membranes, but they are limited to 'static' imaging. Here, we propose a novel method for detailed monitoring of 'dynamic process' of virus capture; specifically, direct examination of biomolecules during filtration under an ultra-stable optical microscope. Samples were fluorescently labeled and infused into a single hollow fiber membrane comprising cuprammonium regenerated-cellulose (Planova 20N). While proteins were able to pa

SUBMITTER: Ayano M 

PROVIDER: S-EPMC7806983 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature