Ontology highlight
ABSTRACT:
SUBMITTER: Yamaguchi K
PROVIDER: S-EPMC7807736 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
ACS omega 20201130 1
We developed adaptive optical (AO) two-photon excitation microscopy by introducing a spatial light modulator (SLM) in a commercially available microscopy system. For correcting optical aberrations caused by refractive index (RI) interfaces at a specimen's surface, spatial phase distributions of the incident excitation laser light were calculated using 3D coordination of the RI interface with a 3D ray-tracing method. Based on the calculation, we applied a 2D phase-shift distribution to a SLM and ...[more]