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Production of benzyl cinnamate by a low-cost immobilized lipase and evaluation of its antioxidant activity and toxicity.

ABSTRACT: In this work was optimized the production of benzyl cinnamate by enzymatic catalysis using the immobilized lipase NS88011 and to evaluate its biological properties. The optimized condition for this system was 1:3 (acid:alcohol) molar ratio, 59?°C, biocatalyst concentration 4.4?mg.mL^-1 for 32?h, with a yield of 97.6 %. The enzyme stability study showed that the enzyme remains active and yields above 60 % until the 13^th cycle (416?h), presenting a promising half-life. In the determination of the antioxidant activity of the ester, an inhibitory concentration necessary to inhibit 50 % of the free radical 2,2-diphenyl-1-picryl-hydrazyl DPPH (IC₅₀) of 149.8?mg.mL^-1 was observed. For acute toxicity against bioindicator Artemia salina, lethal doses (LD₅₀) of 0.07 and 436.7??g.mL^-1 were obtained for the ester and cinnamic acid, showing that benzyl cinnamate had higher toxicity, indicating potential cytotoxic activity against human tumors.

SUBMITTER: Piazza SP

PROVIDER: S-EPMC7809389 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Production of benzyl cinnamate by a low-cost immobilized lipase and evaluation of its antioxidant activity and toxicity.

Piazza Suelen Paloma SP Puton Bruna Maria BM Dallago Rogério Marcos RM de Oliveira Débora D Cansian Rogério Luis RL Mignoni Marcelo M Paroul Natalia N

Biotechnology reports (Amsterdam, Netherlands) 20210106

In this work was optimized the production of benzyl cinnamate by enzymatic catalysis using the immobilized lipase NS88011 and to evaluate its biological properties. The optimized condition for this system was 1:3 (acid:alcohol) molar ratio, 59 °C, biocatalyst concentration 4.4 mg.mL<sup>-1</sup> for 32 h, with a yield of 97.6 %. The enzyme stability study showed that the enzyme remains active and yields above 60 % until the 13<sup>th</sup> cycle (416 h), presenting a promising half-life. In the ...[more]

PMID: 33489787

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Dataset Information

Production of benzyl cinnamate by a low-cost immobilized lipase and evaluation of its antioxidant activity and toxicity.

Publications

Production of benzyl cinnamate by a low-cost immobilized lipase and evaluation of its antioxidant activity and toxicity.

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