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A protocol for rapid pericyte differentiation of human induced pluripotent stem cells.


ABSTRACT: Pericytes play a critical role in promoting, regulating, and maintaining numerous vascular functions. Their dysfunction is a major contributor to the progression of vascular and neurodegenerative diseases, making them an ideal candidate for large-scale production for disease modeling and regenerative cell therapy. This protocol describes the rapid and robust differentiation of pericytes from human induced pluripotent stem cells (hiPSCs) while simultaneously generating a population of hiPSC-derived endothelial progenitor cells. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2017).

SUBMITTER: Aisenbrey EA 

PROVIDER: S-EPMC7811164 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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A protocol for rapid pericyte differentiation of human induced pluripotent stem cells.

Aisenbrey Elizabeth A EA   Torr Elizabeth E   Johnson Hunter H   Soref Cheryl C   Daly William W   Murphy William L WL  

STAR protocols 20210113 1


Pericytes play a critical role in promoting, regulating, and maintaining numerous vascular functions. Their dysfunction is a major contributor to the progression of vascular and neurodegenerative diseases, making them an ideal candidate for large-scale production for disease modeling and regenerative cell therapy. This protocol describes the rapid and robust differentiation of pericytes from human induced pluripotent stem cells (hiPSCs) while simultaneously generating a population of hiPSC-deriv  ...[more]

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