Modulating organelle distribution using light-inducible heterodimerization in C. elegans.
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ABSTRACT:
SUBMITTER: Dansen TB
PROVIDER: S-EPMC7811173 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
Publications
Modulating organelle distribution using light-inducible heterodimerization in <i>C. elegans</i>.
Dansen Tobias B TB De Henau Sasha S
STAR protocols 20210113 1
The relative positioning of organelles underlies fundamental cellular processes, including signaling, polarization, and cellular growth. Here, we describe the usage of a light-dependent heterodimerization system, LOVpep-ePDZ, to alter organelle positioning locally and reversibly in order to study the functional consequences of organelle positioning. The protocol gives details on how to accomplish expression of fusion proteins encoding this system, describes the imaging parameters to achieve subc ...[more]
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