Unknown

Dataset Information

0

Gut Microbiota Contributes to Spontaneous Colitis in E3 Ligase Itch-Deficient Mice.


ABSTRACT: Inflammatory bowel diseases are associated with complex shifts in microbiota composition. However, it remains unclear whether specific subsets of commensal bacteria induce inflammatory bowel diseases in genetically susceptible hosts. In this study, we found that deficiency of the E3 ligase Itch, which leads to spontaneous colitis and rectal prolapse, is associated with alteration of the gut microbiota. 16S rRNA sequencing showed expansion of colitogenic Bacteroides sp. in Itch-/- mice. Treatment with broad-spectrum antibiotics substantially reduced colonic inflammation in Itch-/- mice. Microbiota of Itch-/- mice failed to induce spontaneous colitis upon transfer to Itch+/+ mice but aggravated chemically induced colitis. Furthermore, we found that Bacteroides vulgatus, which is expanded in Itch-/- mice, was sufficient to induce colon inflammation in Itch-/- mice.

SUBMITTER: Kathania M 

PROVIDER: S-EPMC7811274 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

Gut Microbiota Contributes to Spontaneous Colitis in E3 Ligase Itch-Deficient Mice.

Kathania Mahesh M   Tsakem Elviche L EL   Theiss Arianne L AL   Venuprasad K K  

Journal of immunology (Baltimore, Md. : 1950) 20200313 8


Inflammatory bowel diseases are associated with complex shifts in microbiota composition. However, it remains unclear whether specific subsets of commensal bacteria induce inflammatory bowel diseases in genetically susceptible hosts. In this study, we found that deficiency of the E3 ligase Itch, which leads to spontaneous colitis and rectal prolapse, is associated with alteration of the gut microbiota. 16S rRNA sequencing showed expansion of colitogenic <i>Bacteroides</i> sp. in Itch<sup>-/-</su  ...[more]

Similar Datasets

| S-EPMC4583040 | biostudies-literature
| S-EPMC3938399 | biostudies-literature
| S-EPMC4615783 | biostudies-other
| S-EPMC7450106 | biostudies-literature
| S-EPMC2833372 | biostudies-literature
| S-EPMC6344599 | biostudies-literature
| S-EPMC3064387 | biostudies-literature
| S-EPMC6719427 | biostudies-literature
| S-EPMC4063762 | biostudies-literature
2021-05-20 | GSE165789 | GEO