Dataset Information

Pathways involved in pony body size development.

ABSTRACT:

Background

The mechanism of body growth in mammals is poorly understood. Here, we investigated the regulatory networks involved in body growth through transcriptomic analysis of pituitary and epiphyseal tissues of smaller sized Debao ponies and Mongolian horses at the juvenile and adult stages.

Results

We found that growth hormone receptor (GHR) was expressed at low levels in long bones, although growth hormone (GH) was highly expressed in Debao ponies compared with Mongolian horses. Moreover, significant downregulated of the GHR pathway components m-RAS and ATF3 was found in juvenile ponies, which slowed the proliferation of bone osteocytes. However, WNT2 and PLC?2 were obviously upregulated in juvenile Debao ponies, which led to premature mineralization of the bone extracellular matrix. Furthermore, we found that the WNT/Ca²⁺ pathway may be responsible for regulating body growth. GHR was demonstrated by q-PCR and Western blot analyses to be expressed at low levels in long bones of Debao ponies. Treatment with WNT antagonistI decreased the expression of WNT pathway components (P ConclusionOur work provides insights into the genetic regulation of short stature growth in mammals and can provide useful information for the development of therapeutic strategies for small size.

SUBMITTER: Fang J

PROVIDER: S-EPMC7814589 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Publications

Pathways involved in pony body size development.

Fang Jun J Zhang Dong D Cao Jun Wei JW Zhang Li L Liu Chun Xia CX Xing Yan Ping YP Wang Feng F Xu Hong Yang HY Wang Shi Chao SC Ling Yu Y Wang Wei W Zhang Yan Ru YR Zhou Huan Min HM

BMC genomics 20210118 1

<h4>Background</h4>The mechanism of body growth in mammals is poorly understood. Here, we investigated the regulatory networks involved in body growth through transcriptomic analysis of pituitary and epiphyseal tissues of smaller sized Debao ponies and Mongolian horses at the juvenile and adult stages.<h4>Results</h4>We found that growth hormone receptor (GHR) was expressed at low levels in long bones, although growth hormone (GH) was highly expressed in Debao ponies compared with Mongolian hors ...[more]

PMID: 33461495

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Dataset Information

Pathways involved in pony body size development.

Background

Results

Publications

Pathways involved in pony body size development.

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