Unknown

Dataset Information

0

Structural Roles for the Juxtamembrane Linker Region and Transmembrane Region of Synaptobrevin 2 in Membrane Fusion.


ABSTRACT: Formation of the trans-SNARE complex is believed to generate a force transfer to the membranes to promote membrane fusion, but the underlying mechanism remains elusive. In this study, we show that helix-breaking and/or length-increasing insertions in the juxtamembrane linker region of synaptobrevin-2 exert diverse effects on liposome fusion, in a manner dependent on the insertion position relative to the two conserved tryptophan residues (W89/W90). Helical extension of synaptobrevin-2 to W89/W90 is a prerequisite for initiating membrane merger. The transmembrane region of synaptobrevin-2 enables proper localization of W89/W90 at the membrane interface to gate force transfer. Besides, our data indicate that the SNARE regulatory components Munc18-1 and Munc13-1 impose liposome fusion strong demand on tight coupling between the SNARE motif and the transmembrane region of synaptobrevin-2.

SUBMITTER: Hu Y 

PROVIDER: S-EPMC7815645 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

altmetric image

Publications

Structural Roles for the Juxtamembrane Linker Region and Transmembrane Region of Synaptobrevin 2 in Membrane Fusion.

Hu Yaru Y   Zhu Le L   Ma Cong C  

Frontiers in cell and developmental biology 20210106


Formation of the <i>trans</i>-SNARE complex is believed to generate a force transfer to the membranes to promote membrane fusion, but the underlying mechanism remains elusive. In this study, we show that helix-breaking and/or length-increasing insertions in the juxtamembrane linker region of synaptobrevin-2 exert diverse effects on liposome fusion, in a manner dependent on the insertion position relative to the two conserved tryptophan residues (W<sup>89</sup>/W<sup>90</sup>). Helical extension  ...[more]

Similar Datasets

| S-EPMC4388931 | biostudies-literature
| S-EPMC4144707 | biostudies-literature
| S-EPMC4241451 | biostudies-literature
| S-EPMC6500178 | biostudies-literature
| S-EPMC6990648 | biostudies-literature
| S-EPMC9282010 | biostudies-literature
| S-EPMC7412173 | biostudies-literature
| S-EPMC7094234 | biostudies-literature
| S-EPMC3562775 | biostudies-literature
| S-EPMC4249078 | biostudies-literature