Ontology highlight
ABSTRACT:
SUBMITTER: Fay SF
PROVIDER: S-EPMC7816087 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
microPublication biology 20210118
CRISPR/Cas9 genome editing strategies often rely on the placement of an introduced restriction endonuclease (RE) site adjacent to the genomic edit of interest. This allows for rapid initial PCR-based detection of cells and organisms containing the edit of interest and may also be used for subsequent genotyping. Nevertheless, engineering RE sites at optimal locations within coding regions can be difficult due to the many hundreds of potential endonuclease options and the strict requirement to mai ...[more]