Single-step RT-PCR assay for dual genotyping of GI and GII norovirus strains.

ABSTRACT:

Background

Noroviruses are the major cause of acute gastroenteritis (AGE) in people of all ages globally. Standardized genotyping is key for outbreak investigations and surveillance networks.

Objective

Here we describe the validation of a one-step conventional RT-PCR assay for sequence-based dual typing of GI and GII noroviruses. This polymerase (P) and capsid (C) dual typing assay uses a combination of previously published oligonucleotide primers amplifying a genomic region spanning the 3'-end of ORF1 and 5'end of ORF2 resulting in a 579 bp product for GI and 570 bp product for GII viruses.

Results

The limit of detection of the assay ranged from 5 to 50 copies of viral RNA per reaction for GI and GII. To validate the assay, we tested 2,663 noroviruspositive stool sam

SUBMITTER: Chhabra P 

PROVIDER: S-EPMC7816162 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature