Dataset Information

Activated Platelets Convert CD14⁺CD16^- Into CD14⁺CD16⁺ Monocytes With Enhanced Fc?R-Mediated Phagocytosis and Skewed M2 Polarization.

ABSTRACT: Monocytes are important cellular effectors of innate immune defense. Human monocytes are heterogeneous and can be classified into three distinct subsets based on CD14 and CD16 expression. The expansion of intermediate CD14⁺CD16⁺ monocytes has been reported in chronic inflammatory diseases including rheumatoid arthritis (RA). However, the mechanism underlying induction of CD16 and its role in monocytes remains poorly understood. Here, we demonstrate that activated platelets are important for induction of CD16 on classical CD14⁺CD16^- monocytes by soluble factors such as cytokines. Cytokine neutralization and signaling inhibition assays reveal that sequential involvement of platelet-derived TGF-? and monocyte-derived IL-6 contribute to CD16 induction on CD14⁺CD16^- monocytes. Activated platelet-induced CD16 on monocytes participates in antibody-dependent cellular phagocytosis (ADCP) and its level is positively correlated with phagocytic activity. CD14⁺CD16^- monocytes treated with activated platelets preferentially differentiate into M2 macrophages, likely the M2c subset expressing CD163 and MerTK. Lastly, the amount of sCD62P, a marker of activated platelets, is significantly elevated in plasma of RA patients and positively correlates with clinical parameters of RA. Our findings suggest an important role of activated platelets in modulating phenotypical and functional features of human monocytes. This knowledge increases understanding of the immunological role of CD14⁺CD16⁺ cells in chronic inflammatory diseases.

SUBMITTER: Lee SJ

PROVIDER: S-EPMC7817612 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Publications

Activated Platelets Convert CD14+CD16- Into CD14+CD16+ Monocytes With Enhanced FcγR-Mediated Phagocytosis and Skewed M2 Polarization.

Lee Su Jeong SJ Yoon Bo Ruem BR Kim Hee Young HY Yoo Su-Jin SJ Kang Seong Wook SW Lee Won-Woo WW

Frontiers in immunology 20210107

Monocytes are important cellular effectors of innate immune defense. Human monocytes are heterogeneous and can be classified into three distinct subsets based on CD14 and CD16 expression. The expansion of intermediate CD14+CD16+ monocytes has been reported in chronic inflammatory diseases including rheumatoid arthritis (RA). However, the mechanism underlying induction of CD16 and its role in monocytes remains poorly understood. Here, we demonstrate that activated platelets ...[more]

PMID: 33488616

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Project description:BackgroundEvidence on the changes in the absolute counts of monocyte subpopulations in sepsis is missing.MethodsFirstly, absolute counts of circulating CD14pos/HLA-DRpos/CD45pos monocytes were measured by flow cytometry in 70 patients with Gram-negative sepsis and in 10 healthy volunteers. In the second phase, immunophenotyping was performed and the absolute count of circulating inflammatory monocytes and of circulating CD14dim/CD16pos/CD45pos patrolling monocytes were measured in another 55 patients and 10 healthy volunteers. Measurements were repeated on days 3, 7, and 10. Results were correlated with survival after 28 days.ResultsGreater numbers of CD14pos/HLA-DRpos/CD45pos monocytes were found on day 1 in survivors compared to nonsurvivors (p = 0.030). Receiver operating characteristic (ROC) analysis showed that a cutoff higher than 337 cells/mm3 on day 1 could discriminate between survivors and nonsurvivors with a positive predictive value (PPV) of 91.1%. Logistic regression including Sequential Organ Failure Assessment (SOFA) score and Acute Physiology and Chronic Health Evaluation (APACHE) II score showed that an absolute count greater than 337 cells/mm3 was independently associated with unfavorable outcome (odds ratio (OR) 0.19, p = 0.050). The absolute counts of inflammatory and of CD14dim/CD16pos/CD45pos monocytes were greater in patients than healthy controls during the entire 10 days of follow-up. The absolute counts on day 3 of CD14dim/CD16pos/CD45pos monocytes were greater in survivors than nonsurvivors (p = 0.027). ROC analysis revealed that the cutoff at 27 cells/mm3 could discriminate between survivors and nonsurvivors with PPV of 94.1%. Logistic regression including age, SOFA score, and APACHE II score showed that an absolute count greater than 27 cells/mm3 was independently associated with unfavorable outcome (OR 0.06, p = 0.033). Logistic regression analysis showed that intra-abdominal infection on day 1 was predictive of low CD14dim/ CD16pos/CD45pos count on day 3.ConclusionCirculating counts of inflammatory and patrolling monocytes are greatly increased in Gram-negative sepsis. Absolute counts of CD14pos/HLA-DRpos/CD45pos monocytes on day 1 and CD14dim/CD16pos/CD45pos monocytes on day 3 are independently associated with final outcome.Trial registrationClinicalTrials.gov, NCT01223690 . Registered retrospectively on 18 October 2010.

Dataset Information

Activated Platelets Convert CD14⁺CD16^- Into CD14⁺CD16⁺ Monocytes With Enhanced Fc?R-Mediated Phagocytosis and Skewed M2 Polarization.

Publications

Activated Platelets Convert CD14<sup>+</sup>CD16<sup>-</sup> Into CD14<sup>+</sup>CD16<sup>+</sup> Monocytes With Enhanced FcγR-Mediated Phagocytosis and Skewed M2 Polarization.

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Dataset Information

Activated Platelets Convert CD14+CD16- Into CD14+CD16+ Monocytes With Enhanced Fc?R-Mediated Phagocytosis and Skewed M2 Polarization.

Publications

Activated Platelets Convert CD14<sup>+</sup>CD16<sup>-</sup> Into CD14<sup>+</sup>CD16<sup>+</sup> Monocytes With Enhanced FcγR-Mediated Phagocytosis and Skewed M2 Polarization.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Activated Platelets Convert CD14⁺CD16^- Into CD14⁺CD16⁺ Monocytes With Enhanced Fc?R-Mediated Phagocytosis and Skewed M2 Polarization.