Dataset Information

Evaluation of articular cartilage with quantitative MRI in an equine model of post-traumatic osteoarthritis.

ABSTRACT: Chondral lesions lead to degenerative changes in the surrounding cartilage tissue, increasing the risk of developing post-traumatic osteoarthritis (PTOA). This study aimed to investigate the feasibility of quantitative magnetic resonance imaging (qMRI) for evaluation of articular cartilage in PTOA. Articular explants containing surgically induced and repaired chondral lesions were obtained from the stifle joints of seven Shetland ponies (14 samples). Three age-matched nonoperated ponies served as controls (six samples). The samples were imaged at 9.4?T. The measured qMRI parameters included T₁ , T₂ , continuous-wave T_1? (CWT_1? ), adiabatic T_1? (AdT_1? ), and T_2? (AdT_2? ) and relaxation along a fictitious field (T_RAFF ). For reference, cartilage equilibrium and dynamic moduli, proteoglycan content and collagen fiber orientation were determined. Mean values and profiles from full-thickness cartilage regions of interest, at increasing distances from the lesions, were used to compare experimental against control and to correlate qMRI with the references. Significant alterations were detected by qMRI parameters, including prolonged T₁ , CWT_1? , and AdT_1? in the regions adjacent to the lesions. The changes were confirmed by the reference methods. CWT_1? was more strongly associated with the reference measurements and prolonged in the affected regions at lower spin-locking amplitudes. Moderate to strong correlations were found between all qMRI parameters and the reference parameters (??=?-0.531 to -0.757). T₁ , low spin-lock amplitude CWT_1? , and AdT_1? were most responsive to changes in visually intact cartilage adjacent to the lesions. In the context of PTOA, these findings highlight the potential of T₁ , CWT_1? , and AdT_1? in evaluation of compositional and structural changes in cartilage.

SUBMITTER: Kajabi AW

PROVIDER: S-EPMC7818146 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Evaluation of articular cartilage with quantitative MRI in an equine model of post-traumatic osteoarthritis.

Kajabi Abdul Wahed AW Casula Victor V Sarin Jaakko K JK Ketola Juuso H JH Nykänen Olli O Te Moller Nikae C R NCR Mancini Irina A D IAD Visser Jetze J Brommer Harold H René van Weeren P P Malda Jos J Töyräs Juha J Nieminen Miika T MT Nissi Mikko J MJ

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 20200624 1

Chondral lesions lead to degenerative changes in the surrounding cartilage tissue, increasing the risk of developing post-traumatic osteoarthritis (PTOA). This study aimed to investigate the feasibility of quantitative magnetic resonance imaging (qMRI) for evaluation of articular cartilage in PTOA. Articular explants containing surgically induced and repaired chondral lesions were obtained from the stifle joints of seven Shetland ponies (14 samples). Three age-matched nonoperated ponies served a ...[more]

PMID: 32543748

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Project description:Galectins are potent regulators of cell adhesion, growth and apoptosis in diverse cell types, including chondrocytes and synovial fibroblasts. Elevations in synovial fluid galectin-3 have been observed in rheumatoid arthritis, juvenile idiopathic arthritis and experimental inflammatory arthritis in animal models, whereas galectin-1 is thought to be protective. Less is known about galectins-1 and-3 in osteoarthritis (OA). Therefore, the purpose of this study was: (1) to determine whether galectin-1 and-3 synovial fluid concentrations and synovial membrane and cartilage histochemical staining were altered following osteochondral injury in an experimental equine osteoarthritis (OA) model and (2) to measure galectin-1 and-3 mRNA expression and synovial fluid concentrations in naturally occurring equine carpal OA. Synovial fluid galectin-1 and-3 concentrations were quantified using custom ELISAs in two research horse cohorts undergoing experimental OA induction (n = 5 and 4) and in a cohort of horses with naturally occurring carpal OA (n = 57). Galectin mRNA expression in synovial membrane and cartilage tissue obtained from carpal joints of horses with naturally occurring OA was measured using RT-qPCR, and galectin immunostaining was assessed in synovial membrane and osteochondral tissues in the experimental model (n = 5). Synovial fluid galectin-1 and-3 concentrations increased following experimental carpal osteochondral fragmentation. Cartilage galectin-1 mRNA expression increased with OA severity in naturally occurring disease. The superficial zone of healthy articular cartilage stained intensely for galectin-3 in sham-operated joints, whereas galectin-1 staining was nearly absent. Chondrocyte galectin-1 and-3 immunoreactivity increased following cartilage injury, particularly in galectin-1 positive chondrones. Galectins-1 and-3 are present in healthy equine synovial fluid and increase following post-traumatic OA. Healthy superficial zone chondrocytes express galectin-3, whereas galectin-1 chondrocyte staining is limited predominantly to chondrones and injured cartilage. Further work is needed to clarify the functions of galectins-1 and-3 in healthy and OA joints.

Dataset Information

Evaluation of articular cartilage with quantitative MRI in an equine model of post-traumatic osteoarthritis.

Publications

Evaluation of articular cartilage with quantitative MRI in an equine model of post-traumatic osteoarthritis.

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