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Characterization of the complete plastome of Cyperus rotundus L. (Cyperaceae).

ABSTRACT: Cyperus rotundus L. (C. rotundus) is a sedge belonging to the family Cyperaceae and is widely distributed in tropical and warmer temperate regions worldwide. It is one of the oldest traditional medicinal herbs in China, India, Japan, and Korea. In this study, we sequenced the complete chloroplast genome of C. rotundus on the Illumina HiSeq Platform. The chloroplast genome is 182,986 bp in length, with a typical quadripartite structure and consisting of a pair of inverted repeat (IR) regions (35,969 bp) separated by a large single-copy (LSC) region (100,733 bp) and a small single-copy (SSC) region (10,315 bp). It was predicted to contain a total of 133 genes, with an overall GC content of 33.26%. Phylogenetic analysis suggested C. rotundus is sister to Eleocharis celluosa and Eleocharis dulcis.

SUBMITTER: Wu R

PROVIDER: S-EPMC7819115 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:BACKGROUND:Triple-negative breast cancer (TNBC) is a kind of malignant tumor with higher recurrence and metastasis rate. According to historical records, the dry rhizomes Cyperus rotundus L. could be ground into powder and mixed with ginger juice and wine for external application for breast cancer. We studied the effect of the ethanol extract of Cyperus rotundus L. (EECR) on TNBC cells and found its' apoptosis-inducing effect with a dose-relationship. But the function mechanism of EECR on TNBC is still mysterious. Hence, the present research aimed to detect its function mechanism at the small molecule level through ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) metabolomics. METHODS:The CCK-8 assay and the Annexin V-FITC/PI assay were applied to test the effect of EECR on MDA-MB-231 cells and MDA-MB 468 cells at various concentrations of 0, 200, 400, and 600??g/ml. UPLC-Q-TOF-MS/MS based metabolomics was used between the control group and the EECR treatment groups. Multivariate statistical analysis was used to visualize the apoptosis-inducing action of EECR and filtrate significantly changed metabolites. RESULTS:The apoptosis-inducing action was confirmed and forty-nine significantly changed metabolites (VIP >?1, p?<?0.05, and FC >?1.2 or FC <?0.8) were identified after the interference of EECR. The level of significant differential metabolites between control group, middle dose group, and high dose group were compared and found that which supported the apoptosis-inducing action with dose-dependence. CONCLUSION:By means of metabolism, we have detected the mechanism of EECR inducing apoptosis of TNBC cells at the level of small molecule metabolites and found that EECR impacted the energy metabolism of TNBC cells. In addition, we concluded that EECR induced apoptosis by breaking the balance between ATP-production and ATP-consumption: arresting the pathways of Carbohydrate metabolism such as Central carbon metabolism in cancer, aerobic glycolysis, and Amino sugar and nucleotide sugar metabolism, whereas accelerating the pathways of ATP-consumption including Amino Acids metabolism, Fatty acid metabolism, Riboflavin metabolism and Purine metabolism. Although further study is still needed, EECR has great potential in the clinical treatment of TNBC with fewer toxic and side effects.

Dataset Information

Characterization of the complete plastome of Cyperus rotundus L. (Cyperaceae).

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