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Single cell RNA-sequencing data generated from human pluripotent stem cell-derived lens epithelial cells.

ABSTRACT: Detailed transcriptomic analyses of differentiated cell populations derived from human pluripotent stem cells is routinely used to assess the identity and utility of the differentiated cells. Here we provide single cell RNA-sequencing data obtained from ROR1-expressing lens epithelial cells (ROR1e LECs), obtained via directed differentiation of CA1 human embryonic stem cells. Analysis of the data using principal component analysis, heat maps and gene ontology assessments revealed phenotypes associated with lens epithelial cells. These data provide a resource for future characterisation of both normal and cataractous human lens biology. Corresponding morphological and functional data obtained from ROR1e LECs are reported in the associated research article "A simplified method for producing human lens epithelial cells and light-focusing micro-lenses from pluripotent stem cells " (Dewi et al., 2020).

SUBMITTER: Shparberg R

PROVIDER: S-EPMC7820909 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Single cell RNA-sequencing data generated from human pluripotent stem cell-derived lens epithelial cells.

Shparberg Rachel R Dewi Chitra Umala CU Gnanasambandapillai Vikkitharan V Liyanage Liwan L O'Connor Michael D MD

Data in brief 20210108

Detailed transcriptomic analyses of differentiated cell populations derived from human pluripotent stem cells is routinely used to assess the identity and utility of the differentiated cells. Here we provide single cell RNA-sequencing data obtained from ROR1-expressing lens epithelial cells (ROR1e LECs), obtained via directed differentiation of CA1 human embryonic stem cells. Analysis of the data using principal component analysis, heat maps and gene ontology assessments revealed phenotypes asso ...[more]

PMID: 33521174

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